Go Annotations as Summary Text (Tabular View) (GO Graph)

GO curators for mouse genes have assigned the following annotations to the gene product of Dag1. (This text reflects annotations as of Wednesday, January 23, 2013.) MGI curation of this mouse gene is considered complete, including annotations derived from the biomedical literature as of March 26, 2010. If you know of any additional information regarding this mouse gene please let us know. Please supply mouse gene symbol and a PubMed ID.

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Summary from NCBI RefSeq

[Summary is not available for the mouse gene. This summary is for the human ortholog.] Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence. Alternative splicing results in multiple transcript variants all encoding the same protein.[provided by RefSeq, Apr 2010]

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Summary text based on GO annotations supported by experimental evidence in mouse

• Researchers have inferred from direct assay, that the gene product of Dag1
  • is located in the following cellular components:
    • basement membrane ^{[1, 2]}
    • cell outer membrane ^[8]
    • dystroglycan complex ^[1]
    • membrane raft ^[3]
    • plasma membrane ^{[5, 10, 11]}
    • sarcolemma ^{[3, 4, 6]}
• The gene product of Dag1 has been shown to bind to the gene products of Dag1, Egflam, Lama1. ^{[1, 7, 9, 10, 11]}
• Researchers have inferred, based on phenotypic analysis of mouse mutants, that the gene product of Dag1
  • participates in the following biological processes:
    • branching involved in salivary gland morphogenesis ^[1]
    • epithelial tube branching involved in lung morphogenesis ^[1]
    • morphogenesis of an epithelial sheet ^[1]
    • myelination in peripheral nervous system ^[8]
    • nerve maturation ^[8]
    • Schwann cell development ^[8]
  • is located in the following cellular components:
    • node of Ranvier ^[8]

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Summary text based on GO annotations supported by experimental evidence in other organisms

• From comparative sequence analysis (see table for details), MGI curators have determined that the gene product of Dag1:
  • participates in the following biological processes:
    • calcium-dependent cell-matrix adhesion
    • cytoskeletal anchoring at plasma membrane
    • membrane protein ectodomain proteolysis
    • microtubule anchoring
    • negative regulation of cell migration
    • negative regulation of MAPK cascade
    • negative regulation of protein kinase B signaling cascade
    • NLS-bearing substrate import into nucleus
    • virus-host interaction
  • performs the following molecular functions:
    • actin binding
    • alpha-actinin binding
    • bacterial cell surface binding
    • protein complex binding
    • SH2 domain binding
    • structural constituent of muscle
    • tubulin binding
    • vinculin binding
  • is located in the following cellular components:
    • basement membrane
    • basolateral plasma membrane
    • cell-cell adherens junction
    • contractile ring
    • costamere
    • cytoplasm
    • dystrophin-associated glycoprotein complex
    • extracellular space
    • filopodium
    • focal adhesion
    • integral to membrane
    • lamellipodium
    • nucleoplasm
    • plasma membrane
    • sarcolemma

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Summary text based on GO annotations supported by structural data

• Dag1 encodes a protein or proteins that contain the following InterPro domains: Cadherin-like, Dystroglycan, Dystroglycan-type cadherin-like, Immunoglobulin-like fold, indicating that the gene product:
  • performs the following molecular functions:
    • calcium ion binding
  • is located in the following cellular components:
    • membrane

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Summary text for additional MGI annotations

• Automated translation to GO terms of SwissProt keywords that describe Dag1 indicates that it:
  • is located in the following cellular components:
    • cell junction
    • cytoskeleton
    • extracellular region
    • membrane
    • nucleus
    • postsynaptic membrane
    • synapse

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References

1. Durbeej M et al. (2001) Dystroglycan binding to laminin alpha1LG4 module influences epithelial morphogenesis of salivary gland and lung in vitro. Differentiation, 69:121-34. (PubMed:11798066)
2. Durbeej M et al. (1998) Distribution of dystroglycan in normal adult mouse tissues. J Histochem Cytochem, 46:449-57. (PubMed:9524190)
3. Galbiati F et al. (2001) Caveolin-3 null mice show a loss of caveolae, changes in the microdomain distribution of the dystrophin-glycoprotein complex, and t-tubule abnormalities. J Biol Chem, 276:21425-33. (PubMed:11259414)
4. Hagiwara Y et al. (2000) Caveolin-3 deficiency causes muscle degeneration in mice Hum Mol Genet, 9:3047-54. (PubMed:11115849)
5. Koh TJ et al. (2004) Cytoskeletal disruption and small heat shock protein translocation immediately after lengthening contractions. Am J Physiol Cell Physiol, 286:C713-22. (PubMed:14627610)
6. Liang B et al. (1998) Injection of T-cell receptor peptide reduces immunosenescence in aged C57BL/6 mice. Immunology, 93:462-8. (PubMed:9659216)
7. Rentschler S et al. (1999) The WW domain of dystrophin requires EF-hands region to interact with beta-dystroglycan. Biol Chem, 380:431-42. (PubMed:10355629)
8. Saito F et al. (2003) Unique role of dystroglycan in peripheral nerve myelination, nodal structure, and sodium channel stabilization. Neuron, 38:747-58. (PubMed:12797959)
9. Sato S et al. (2008) Pikachurin, a dystroglycan ligand, is essential for photoreceptor ribbon synapse formation. Nat Neurosci, 11:923-31. (PubMed:18641643)
10. Sherman DL et al. (2001) Specific disruption of a schwann cell dystrophin-related protein complex in a demyelinating neuropathy. Neuron, 30:677-87. (PubMed:11430802)
11. Sotgia F et al. (2000) Caveolin-3 directly interacts with the C-terminal tail of beta -dystroglycan. IDENTIFICATION OF A CENTRAL WW-LIKE DOMAIN WITHIN CAVEOLIN FAMILY MEMBERS J Biol Chem, 275:38048-58. (PubMed:10988290)

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Gene Ontology Evidence Code Abbreviations:

EXP Inferred from experiment

IC Inferred by curator

IDA Inferred from direct assay

IEA Inferred from electronic annotation

IGI Inferred from genetic interaction

IMP Inferred from mutant phenotype

IPI Inferred from physical interaction

ISS Inferred from sequence or structural similarity

ISO Inferred from sequence orthology

ISA Inferred from sequence alignment

ISM Inferred from sequence model

NAS Non-traceable author statement

ND No biological data available

RCA Reviewed computational analysis

TAS Traceable author statement

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