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Gene Expression Data
Assay Details
Assay
Reference: J:115783 Kiernan AE, et al., The Notch ligand JAG1 is required for sensory progenitor development in the mammalian inner ear. PLoS Genet. 2006 Jan;2(1):e4
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6763028
Gene symbol: S100a1
Gene name: S100 calcium binding protein A1
Antibody: Anti-S100A1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 6A: embryonic day 18.5 (more )
Note: Apex region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6A Expressed in inner hair cells, Dieters cells, and inner phalangeal cells.

Specimen 6B: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Apex region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6B Expressed in inner hair cells, Dieters cells, and inner phalangeal cells. Outer hair cells were not present in the mutant.

Specimen 6C: embryonic day 18.5 (more )
Note: Middle region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6C Expressed in inner hair cells, Dieters cells, and inner phalangeal cells.

Specimen 6D: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Middle region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6D Expressed in inner hair cells, Dieters cells, and inner phalangeal cells, although their patterning was clearly abnormal.

Specimen 6E: embryonic day 18.5 (more )
Note: Base region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6E Expressed in inner hair cells, Dieters cells, and inner phalangeal cells.

Specimen 6F: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Base region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: cochlea epithelium Present Regionally restricted 6F No hair cells were present in the base region.

Specimen 6G: embryonic day 18.5 (more )
Note: Utricle region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: utricle epithelium Present Regionally restricted 6G Expressed in inner hair cells, Dieters cells, and inner phalangeal cells.

Specimen 6H: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Utricle region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: utricle epithelium Present Regionally restricted 6H Expressed in inner hair cells, Dieters cells, and inner phalangeal cells. The utricular macula is extremely small with very few positive hair cells.

Specimen 6I: embryonic day 18.5 (more )
Note: Saccule region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: saccule epithelium Present Regionally restricted 6I Expressed in inner hair cells, Dieters cells, and inner phalangeal cells.

Specimen 6J: embryonic day 18.5; Jag1tm1Grid/Jag1tm2Grid, Foxg1tm1(cre)Skm/Foxg1+ (more )
Note: Conditional mutant. Saccule region. Double labeled: green - S100a1; red - Myo7a (assay MGI:6763027).
Structure Level Pattern Image Note
TS26: saccule epithelium Present Regionally restricted 6J Expressed in inner hair cells, Dieters cells, and inner phalangeal cells. Hair cell differentiation appeared relatively unaffected, although the entire saccular structure was shaped differently than in the controls.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2022
MGI 6.20
The Jackson Laboratory