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Gene Expression Data
Assay Details
Assay
Reference: J:43803 Baskaran K, et al., Cloning and developmental expression of the murine homolog of the acute leukemia proto-oncogene AF4. Oncogene. 1997 Oct 16;15(16):1967-78
Assay type: RNA in situ
MGI Accession ID: MGI:6507839
Gene symbol: Aff1
Gene name: AF4/FMR2 family, member 1
Probe: Af4 cDNA2
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen 6a: postnatal adult (more )
Structure Level Pattern Image Note
TS28: spleen red pulp Very strong Regionally restricted 6b , 6a Expression is in the myeloid, erthyroid and megakaryocytic lineages.
TS28: spleen white pulp Present 6b , 6a
TS28: spleen marginal zone Weak 6a , 6b
TS28: spleen Present Regionally restricted 6b , 6a There is uniform strong expression in T-cells adjacent to vessels.
TS28: spleen germinal center Present Non-Uniform 6a , 6b
TS28: spleen follicular dendritic cell network Very strong Regionally restricted 6a , 6b

Specimen 7b: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo mesoderm Strong 7b
TS11: embryonic visceral endoderm Strong 7b
TS11: parietal endoderm Strong 7b
TS11: ectoplacental cone Weak 7b
TS11: neural plate Weak 7b
TS11: trophectoderm Present Regionally restricted 7b Expression is in invading trophoblasts.
TS11: extraembryonic visceral endoderm Absent 7b

Specimen 8b: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: embryo Present 8b
TS13: trophectoderm Present Regionally restricted 8b Expression is in invading trophoblasts.

Specimen 8d: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS13: blood island Present Regionally restricted 8d Expression is in some hematopoietic cells and in all endothelial cells of the blood islands.

Specimen 9b: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: blood vessel endothelium Strong 9b
TS17: cardiac muscle tissue Strong 9b
TS17: endocardial lining Strong 9b
TS17: dorsal aorta Strong Regionally restricted 9b Expression is in the endothelium of the aorta.
TS17: umbilical vein embryonic part Strong Regionally restricted 9b Expression is in the endothelium of the umbilical vein.

Specimen 9d: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: heart Weak 9d

Specimen 10b: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: arch of aorta Weak 10b
TS22: artery endothelium Weak 10b

Specimen 10d: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: perichondrium Present Regionally restricted 10d Expression is in the perichondrium of the vertebral bodies.
TS23: vertebra Present Regionally restricted 10d Expression is in the perichondral cells with marked down- regulation in the chondrocytes.

Specimen 10f: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: pituitary gland Present 10f

Specimen not shown Adult: postnatal adult (more )
Structure Level Pattern Image Note
TS28: liver Strong Regionally restricted Expression is in hepatocytes.
TS28: liver sinusoid Weak Regionally restricted Expression is in the endothelium.
TS28: medullary tubule Present Regionally restricted Expression is in the epithelia.
TS28: alimentary system lamina propria Present Regionally restricted Expression is in lymphocytes.
TS28: alimentary system smooth muscle Present
TS28: pancreatic acinus Present
TS28: renal cortical arterial system Present
TS28: alimentary system epithelium Absent
TS28: glomerular capillary endothelium Absent
TS28: islets of Langerhans Absent
TS28: pancreatic duct Absent

Specimen not shown E9.5: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: embryo Present
TS15: mesenchyme derived from splanchnopleure Present
TS15: extraembryonic component Present Regionally restricted Expression is in the extraembryonic membranes.
TS15: trophectoderm Present Regionally restricted Expression is in invading trophoblasts.

Specimen not shown 10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: sclerotome Absent

Specimen not shown 11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: alimentary system epithelium Present
TS19: hindlimb bud Present
TS19: retina Present
TS19: sclerotome Present
TS19: Rathke's pouch Present Widespread
TS19: central nervous system Present Widespread
TS19: alimentary system Present Regionally restricted Expression is in the smooth muscle precursors.
TS19: nephric duct Present Regionally restricted Expression is in the epithelium.
TS19: lung epithelium Present Regionally restricted Expression is in the bronchiolar epithelium.

Specimen not shown 12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: alimentary system epithelium Present
TS20: hindlimb Present
TS20: retina Present
TS20: sclerotome Present
TS20: central nervous system Present Widespread
TS20: remnant of Rathke's pouch Present Widespread
TS20: alimentary system Present Regionally restricted Expression is in the smooth muscle precursors.
TS20: smooth muscle tissue Present Regionally restricted Expression is in the smooth muscle precursors in the gastrointestinal system.
TS20: nephric duct Present Regionally restricted Expression is in the epithelium.
TS20: lung epithelium Present Regionally restricted Expression is in the bronchiolar epithelium.
TS20: heart Weak

Specimen not shown 13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: vertebral cartilage condensation Strong Regionally restricted Expression is in chondrocytes.
TS21: alimentary system epithelium Strong
TS21: alimentary system smooth muscle Strong
TS21: lamina propria Strong
TS21: perichondrium Strong
TS21: central nervous system Present
TS21: dorsal root ganglion Present
TS21: skin Present
TS21: liver Present Expression is in both the hepatocytes and hematopoietic tissue.
TS21: lung epithelium Strong Regionally restricted Expression is in the bronchiolar epithelium.
TS21: lung Weak Regionally restricted Expression is in the pulmonary parenchyma.
TS21: pancreas Weak

Specimen not shown 14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: alimentary system smooth muscle Present
TS22: skeletal muscle Present
TS22: skin Present
TS22: lung Present Regionally restricted Expression is in the lung parenchyma and is down-regulated in the bronchiolar epithelium.
TS22: alimentary system epithelium Weak

Specimen not shown 15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: hair follicle Present Regionally restricted Expression is in the basal cells of the hair follicles.

Specimen not shown 17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: skeletal muscle Strong Expression is in skeletal muscle and is absent in fibroblasts.
TS25: perichondrium Strong Regionally restricted Expression is in perichondral cells of the vertebral bodies.
TS25: vertebra Strong Regionally restricted Expression is in perichondral cells of the vertebral bodies.
TS25: cartilage Strong Regionally restricted Expression is in chondrocytes.
TS25: central nervous system Present Expression is in glia and neurons.
TS25: hematopoietic system Present Regionally restricted The majority of the hepatic hematopoietic cells are strongly positive whereas the hepatocytes are weakly positive.
TS25: liver Present Regionally restricted The majority of the hepatic hematopoietic cells are strongly positive whereas the hepatocytes are weakly positive.
TS25: skin Present Regionally restricted Expression is in the basal layer and expression is down-regulated in the more differentiated layers.
TS25: alimentary system Present Regionally restricted Expression is in some lymphocytes.
TS25: alimentary system smooth muscle Present
TS25: lung alveolus Weak
TS25: cardiac muscle tissue Absent
TS25: lung vascular element Absent Expression is absent in the pulmonary endothelia.

Specimen Table 1 E10.5: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: heart Strong The peak of expression during development of this tissue is at this age.
TS17: brain Strong

Specimen Table 1 E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: heart Present Expression in this tissue starts to down-regulate at this age.
TS19: brain Strong

Specimen Table 1 E13.5: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: alimentary system epithelium Strong The peak of expression during development of this tissue is at this age.
TS21: axial skeleton Strong The peak of expression during development of this tissue is at this age.
TS21: lung Strong The peak of expression during development of this tissue is at this age.
TS21: brain Strong

Specimen Table 1 E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: skin Strong The peak of expression during development of this tissue is at this age.
TS22: alimentary system epithelium Present Expression in this tissue starts to down-regulate at this age.
TS22: lung Present Expression in this tissue starts to down-regulate at this age.
TS22: brain Strong

Specimen Table 1 E15.5: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: axial skeleton Present Expression in this tissue starts to down-regulate at this age.
TS23: brain Strong

Specimen Table 1 E17.5: embryonic day 17.5 (more )
Structure Level Pattern Image Note
TS25: skin Present Expression in this tissue starts to down-regulate in the suprabsal layers at this age.
TS25: brain Strong


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/05/2021
MGI 6.17
The Jackson Laboratory