Probe:	Af4 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Very strong	Regionally restricted	6b , 6a	Expression is in the myeloid, erthyroid and megakaryocytic lineages.
TS28: spleen white pulp	Present		6b , 6a
TS28: spleen marginal zone	Weak		6a , 6b
TS28: spleen	Present	Regionally restricted	6b , 6a	There is uniform strong expression in T-cells adjacent to vessels.
TS28: spleen germinal center	Present	Non-Uniform	6a , 6b
TS28: spleen follicular dendritic cell network	Very strong	Regionally restricted	6a , 6b

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Strong		7b
TS11: embryonic visceral endoderm	Strong		7b
TS11: parietal endoderm	Strong		7b
TS11: ectoplacental cone	Weak		7b
TS11: neural plate	Weak		7b
TS11: trophectoderm	Present	Regionally restricted	7b	Expression is in invading trophoblasts.
TS11: extraembryonic visceral endoderm	Absent		7b

Structure	Level	Pattern	Image	Note
TS13: embryo	Present		8b
TS13: trophectoderm	Present	Regionally restricted	8b	Expression is in invading trophoblasts.

Structure	Level	Pattern	Image	Note
TS13: blood island	Present	Regionally restricted	8d	Expression is in some hematopoietic cells and in all endothelial cells of the blood islands.

Structure	Level	Pattern	Image	Note
TS17: blood vessel endothelium	Strong		9b
TS17: cardiac muscle tissue	Strong		9b
TS17: endocardial lining	Strong		9b
TS17: dorsal aorta	Strong	Regionally restricted	9b	Expression is in the endothelium of the aorta.
TS17: umbilical vein embryonic part	Strong	Regionally restricted	9b	Expression is in the endothelium of the umbilical vein.

Structure	Level	Pattern	Image	Note
TS19: heart	Weak		9d

Structure	Level	Pattern	Image	Note
TS22: arch of aorta	Weak		10b
TS22: artery endothelium	Weak		10b

Structure	Level	Pattern	Image	Note
TS23: perichondrium	Present	Regionally restricted	10d	Expression is in the perichondrium of the vertebral bodies.
TS23: vertebra	Present	Regionally restricted	10d	Expression is in the perichondral cells with marked down- regulation in the chondrocytes.

Structure	Level	Pattern	Image	Note
TS25: pituitary gland	Present		10f

Structure	Level	Pattern	Image	Note
TS28: liver	Strong	Regionally restricted		Expression is in hepatocytes.
TS28: liver sinusoid	Weak	Regionally restricted		Expression is in the endothelium.
TS28: medullary tubule	Present	Regionally restricted		Expression is in the epithelia.
TS28: alimentary system lamina propria	Present	Regionally restricted		Expression is in lymphocytes.
TS28: alimentary system smooth muscle	Present
TS28: pancreatic acinus	Present
TS28: renal cortical arterial system	Present
TS28: alimentary system epithelium	Absent
TS28: glomerular capillary endothelium	Absent
TS28: islets of Langerhans	Absent
TS28: pancreatic duct	Absent

Structure	Level	Pattern	Image	Note
TS15: embryo	Present
TS15: mesenchyme derived from splanchnopleure	Present
TS15: extraembryonic component	Present	Regionally restricted		Expression is in the extraembryonic membranes.
TS15: trophectoderm	Present	Regionally restricted		Expression is in invading trophoblasts.

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Absent

Structure	Level	Pattern	Image	Note
TS19: alimentary system epithelium	Present
TS19: hindlimb bud	Present
TS19: retina	Present
TS19: sclerotome	Present
TS19: Rathke's pouch	Present	Widespread
TS19: central nervous system	Present	Widespread
TS19: alimentary system	Present	Regionally restricted		Expression is in the smooth muscle precursors.
TS19: nephric duct	Present	Regionally restricted		Expression is in the epithelium.
TS19: lung epithelium	Present	Regionally restricted		Expression is in the bronchiolar epithelium.

Structure	Level	Pattern	Image	Note
TS20: alimentary system epithelium	Present
TS20: hindlimb	Present
TS20: retina	Present
TS20: sclerotome	Present
TS20: central nervous system	Present	Widespread
TS20: remnant of Rathke's pouch	Present	Widespread
TS20: alimentary system	Present	Regionally restricted		Expression is in the smooth muscle precursors.
TS20: smooth muscle tissue	Present	Regionally restricted		Expression is in the smooth muscle precursors in the gastrointestinal system.
TS20: nephric duct	Present	Regionally restricted		Expression is in the epithelium.
TS20: lung epithelium	Present	Regionally restricted		Expression is in the bronchiolar epithelium.
TS20: heart	Weak

Structure	Level	Pattern	Image	Note
TS21: vertebral cartilage condensation	Strong	Regionally restricted		Expression is in chondrocytes.
TS21: alimentary system epithelium	Strong
TS21: alimentary system smooth muscle	Strong
TS21: lamina propria	Strong
TS21: perichondrium	Strong
TS21: central nervous system	Present
TS21: dorsal root ganglion	Present
TS21: skin	Present
TS21: liver	Present			Expression is in both the hepatocytes and hematopoietic tissue.
TS21: lung epithelium	Strong	Regionally restricted		Expression is in the bronchiolar epithelium.
TS21: lung	Weak	Regionally restricted		Expression is in the pulmonary parenchyma.
TS21: pancreas	Weak

Structure	Level	Pattern	Image	Note
TS22: alimentary system smooth muscle	Present
TS22: skeletal muscle	Present
TS22: skin	Present
TS22: lung	Present	Regionally restricted		Expression is in the lung parenchyma and is down-regulated in the bronchiolar epithelium.
TS22: alimentary system epithelium	Weak

Structure	Level	Pattern	Image	Note
TS23: hair follicle	Present	Regionally restricted		Expression is in the basal cells of the hair follicles.

Structure	Level	Pattern	Image	Note
TS25: skeletal muscle	Strong			Expression is in skeletal muscle and is absent in fibroblasts.
TS25: perichondrium	Strong	Regionally restricted		Expression is in perichondral cells of the vertebral bodies.
TS25: vertebra	Strong	Regionally restricted		Expression is in perichondral cells of the vertebral bodies.
TS25: cartilage	Strong	Regionally restricted		Expression is in chondrocytes.
TS25: central nervous system	Present			Expression is in glia and neurons.
TS25: hematopoietic system	Present	Regionally restricted		The majority of the hepatic hematopoietic cells are strongly positive whereas the hepatocytes are weakly positive.
TS25: liver	Present	Regionally restricted		The majority of the hepatic hematopoietic cells are strongly positive whereas the hepatocytes are weakly positive.
TS25: skin	Present	Regionally restricted		Expression is in the basal layer and expression is down-regulated in the more differentiated layers.
TS25: alimentary system	Present	Regionally restricted		Expression is in some lymphocytes.
TS25: alimentary system smooth muscle	Present
TS25: lung alveolus	Weak
TS25: cardiac muscle tissue	Absent
TS25: lung vascular element	Absent			Expression is absent in the pulmonary endothelia.

Structure	Level	Pattern	Image	Note
TS17: heart	Strong			The peak of expression during development of this tissue is at this age.
TS17: brain	Strong

Structure	Level	Pattern	Image	Note
TS19: heart	Present			Expression in this tissue starts to down-regulate at this age.
TS19: brain	Strong

Structure	Level	Pattern	Image	Note
TS21: alimentary system epithelium	Strong			The peak of expression during development of this tissue is at this age.
TS21: axial skeleton	Strong			The peak of expression during development of this tissue is at this age.
TS21: lung	Strong			The peak of expression during development of this tissue is at this age.
TS21: brain	Strong

Structure	Level	Pattern	Image	Note
TS22: skin	Strong			The peak of expression during development of this tissue is at this age.
TS22: alimentary system epithelium	Present			Expression in this tissue starts to down-regulate at this age.
TS22: lung	Present			Expression in this tissue starts to down-regulate at this age.
TS22: brain	Strong

Structure	Level	Pattern	Image	Note
TS23: axial skeleton	Present			Expression in this tissue starts to down-regulate at this age.
TS23: brain	Strong

Structure	Level	Pattern	Image	Note
TS25: skin	Present			Expression in this tissue starts to down-regulate in the suprabsal layers at this age.
TS25: brain	Strong