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Gene Expression Data
Assay Details
Assay
Reference: J:77889 Britanova O, et al., The Mouse Laf4 Gene: Exon/Intron Organization, cDNA Sequence, Alternative Splicing, and Expression during Central Nervous System Development. Genomics. 2002 Jul;80(1):31-7
Assay type: RNA in situ
MGI Accession ID: MGI:6403248
Gene symbol: Aff3
Gene name: AF4/FMR2 family, member 3
Probe: Laf4 cDNA3
Probe preparation: Antisense RNA labelled with S35
Visualized with: Autoradiography
Assay notes: Two probes were used in these studies, one to the coding region and one to the non-coding region. Both produced the same results.
Results
Specimen 3A/B: embryonic day 13.5 (more )
Note: transverse section
Structure Level Pattern Image Note
TS21: cerebellum Present 3A/B This is a main site of expression.
TS21: cerebral cortex Present 3A/B This is a main site of expression.
TS21: thalamus Present 3A/B This is a main site of expression.
TS21: hypothalamus Present 3A/B Lesser expression is seen in this site.
TS21: midbrain Present 3A/B Lesser expression is seen in this site.
TS21: cerebral cortex subventricular zone Present 3A/B Most expression in the cortex is confined here.

Specimen 3C/D: embryonic day 15.5 (more )
Note: frontal section
Structure Level Pattern Image Note
TS23: cerebral cortex subventricular zone Present 3C/D
TS23: cortical plate Present 3C/D
TS23: amygdala Present 3C/D
TS23: thalamus Present Non-Uniform 3C/D Stronger expression is seen in the ventral part of the thalamus; weak expression is seen in the remainder.

Specimen 3E/F: embryonic day 16.5 (more )
Note: frontal section
Structure Level Pattern Image Note
TS24: cortical plate Present 3E/F

Specimen 3G/H: postnatal day 14 (more )
Note: frontal section
Structure Level Pattern Image Note
TS28: dentate gyrus Present 3G/H
TS28: hippocampus Present 3G/H
TS28: piriform cortex Present 3G/H

Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: amygdala Present
TS28: hippocampus Present
TS28: piriform cortex Present

Specimen 4A/B: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cerebellum Present 4A/B This is a main site of expression.
TS21: cerebral cortex Present 4A/B This is a main site of expression.
TS21: thalamus Present 4A/B This is a main site of expression.
TS21: hypothalamus Present 4A/B Lesser expression is seen in this site.
TS21: midbrain Present 4A/B Lesser expression is seen in this site.
TS21: limb cartilage condensation Present 4A/B
TS21: rib cartilage condensation Present 4A/B
TS21: vertebral cartilage condensation Present 4A/B
TS21: nasal septum Present 4A/B Expression is seen in the cartilage.
TS21: cochlea Present 4A/B
TS21: lung Present 4A/B
TS21: primitive bladder Present 4A/B
TS21: renal tubule Present 4A/B
TS21: heart Absent 4A/B
TS21: liver Absent 4A/B
TS21: thymus primordium Absent 4A/B

Specimen 4D/E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cerebral cortex Present 4D/E Expression is seen in migrating cells.
TS26: cerebral cortex intermediate zone Present 4D/E
TS26: basal ganglia Absent 4D/E
TS26: inferior colliculus Present 4D/E
TS26: superior colliculus Absent 4D/E

Specimen 5A/B: embryonic day 18.5 (more )
Note: frontal section
Structure Level Pattern Image Note
TS26: cerebral cortex Present 5A/B
TS26: piriform cortex Present 5A/B

Specimen 5C/D: embryonic day 18.5; Pax6Sey/Pax6Sey (more )
Note: frontal section
Structure Level Pattern Image Note
TS26: cerebral cortex Present 5C/D In the mutant expression is downregulated in the lateroventral part of the cerebral cortex.
TS26: piriform cortex Absent 5C/D

Specimen 5E: postnatal day 2 (more )
Note: frontal section
Structure Level Pattern Image Note
TS27: cerebral cortex Present Graded 5E There is a gradient of expression from upper to deep layers.

Specimen 5F: postnatal day 2; Relnrl/Relnrl (more )
Note: frontal section
Structure Level Pattern Image Note
TS27: cerebral cortex Present Graded 5F In the mutant there is a gradient of expression from deep to upper layers, the inverse of what is seen in wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory