Nefh Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:284681 Ivankovic D, et al., Axonal autophagosome maturation defect through failure of ATG9A sorting underpins pathology in AP-4 deficiency syndrome. Autophagy. 2020 Mar;16(3):391-407
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6402249
Gene symbol:	Nefh
Gene name:	neurofilament, heavy polypeptide

Antibody:	Anti-Nefh (ab4680)
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

1g WT ©

Specimen 1g WT: postnatal month 1 (more

)

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		1g WT

1g KO ©

Specimen 1g KO: postnatal month 1; Ap4e1^{tm1b(KOMP)Wtsi}/Ap4e1^{tm1b(KOMP)Wtsi} (more

)

Structure	Level	Pattern	Image	Note
TS28: corpus callosum	Present		1g KO

2d WT NF200 ©

2e WT NF200 ©

2f WT NF200 ©

Specimen 2d WT: postnatal month 1 (more

)
Note: Double labeled: blue - Nefh; red - Atg9a (assay MGI:6402274).

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2e WT NF200 , 2d WT NF200
TS28: hippocampus CA1	Present		2d WT NF200 , 2f WT NF200

2d KO NF200 ©

2e KO NF200 ©

2f KO NF200 ©

Specimen 2d KO: postnatal month 1; Ap4e1^{tm1b(KOMP)Wtsi}/Ap4e1^{tm1b(KOMP)Wtsi} (more

)
Note: Double labeled: blue - Nefh; red - Atg9a (assay MGI:6402274).

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		2e KO NF200 , 2d KO NF200
TS28: hippocampus CA1	Present		2f KO NF200 , 2d KO NF200

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/16/2024 MGI 6.23