Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:74163
El-Amraoui A, et al., Spatiotemporal expression of otogelin in the developing and adult mouse inner ear. Hear Res. 2001 Aug;158(1-2):151-9
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6277928
|
|
Gene symbol:
|
Otog
|
|
Gene name:
|
otogelin
|
|
Antibody:
|
antiserum 7.8
|
|
Detection system:
|
Secondary antibody coupled to fluorescein
|
|
Assay notes:
|
Either this antibody or another one (antiserum 3.4) was used.
|
|
|
Results
|
Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 10.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst epithelium |
Present |
Punctate |
1A
|
Expressed in various areas of the pluristratified epithelium.
|
Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: otocyst epithelium |
Strong |
Regionally restricted |
1C
|
Increased expression, with the intense signal located in the ventro-medial epithelium close to the sag.
|
Specimen
1E:
embryonic day 12.5
(more )
Specimen
1E:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
1G:
embryonic day 12.5
(more )
Specimen
1G:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 12.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea epithelium |
Present |
|
1G
|
Expressed in the emerging cochlear duct corresponded to the presumptive areas of non-sensory cells.
|
Specimen
2A:
embryonic day 13.5
(more )
Specimen
2A:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: saccule |
Present |
|
2A
|
Expressed in the non-sensory cells of the developing vestibule.
|
| TS21: utricle |
Present |
|
2A
|
Expressed in the non-sensory cells of the developing vestibule.
|
| TS21: semicircular canal |
Present |
Regionally restricted |
2A
|
Expressed in crista ampularis epithelium. Expressed in the non-sensory cells of the developing vestibule.
|
Specimen
2B:
embryonic day 13.5
(more )
Specimen
2B:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2C:
embryonic day 15.5
(more )
Specimen
2C:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2D:
embryonic day 16.5
(more )
Specimen
2D:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: semicircular canal |
Strong |
Regionally restricted |
2D
|
Expressed in the crista ampularis and the cupula.
|
Specimen
2E:
postnatal day 60
(more )
Specimen
2E:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
2F:
postnatal day 60
(more )
Specimen
2F:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: crista ampullaris |
Present |
Regionally restricted |
2F
|
Expressed in the supporting cells and in the transitory epithelium. Expressed in the cupula.
|
Specimen
2G:
postnatal day 60
(more )
Specimen
2G:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: utricle epithelium |
Present |
|
2G
|
Expressed in the supporting cells and in the transitory epithelium. Expressed in the otoconial membrane.
|
Specimen
2H:
postnatal day 60
(more )
Specimen
2H:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 60
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: utricle epithelium |
Present |
|
2H
|
Expressed in the supporting cells and in the transitory epithelium. Expressed in the otoconial membrane.
|
Specimen
3A:
postnatal day 0
(more )
Specimen
3A:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: macula of utricle |
Present |
Widespread |
3A
|
Expressed throughout the cell cytoplasm of the supporting cells.
|
Specimen
3B:
postnatal day 8
(more )
Specimen
3B:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: macula of utricle |
Present |
|
3B
|
Gradually decreased expression within the supporting cells following a basal to apical gradient.
|
Specimen
3C:
postnatal day 20
(more )
Specimen
3C:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 20
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: macula of utricle |
Present |
Regionally restricted |
3C
|
Restricted to the apical part of the supporting cells, while in the above otoconial membranes, the labeling became broader
|
Specimen
5A:
embryonic day 16.5
(more )
Specimen
5A:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 16.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: cochlear duct |
Strong |
Regionally restricted |
5A
|
Expressed in all the non-sensory cells, the interdental cells of the spiral limbus and in the tectorial membrane. Also expressed in Claudius cells, the spiral prominence and Reissner's membrane.
|
| TS24: greater epithelial ridge |
Present |
|
5A
|
Expressed in the columnar cells.
|
Specimen
5B:
embryonic day 18.5
(more )
Specimen
5B:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tectorial membrane |
Strong |
|
5B
|
Expressed in the major and minor tectorial membranes.
|
| TS26: organ of Corti |
Present |
|
5B
|
Expressed in the non-sensory cells, i.e. pillar, Deiter's and Hensen's cells. Also expressed in Claudius cells, the spiral prominence and Reissner's membrane.
|
| TS26: cochlear duct |
Present |
Regionally restricted |
5B
|
Expressed in the interdental cells of the spiral limbus.
|
| TS26: greater epithelial ridge |
Present |
|
5B
|
Expressed in the columnar cells.
|
Specimen
5C:
postnatal day 2
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 2
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: tectorial membrane |
Strong |
|
5C
|
Expressed in the major and minor tectorial membranes.
|
| TS27: spiral limbus |
Present |
|
5C
|
Expressed in the interdental cells of the spiral limbus.
|
| TS27: cochlea epithelium |
Present |
Regionally restricted |
5C
|
Expressed in the columnar cells that form the greater epithelial ridge. Decreased expression in the spiral prominence, the Reissner's membrane, and Claudius cells. Expressed in the pillar cells and Hensen's cells.
|
Specimen
5D:
postnatal day 4
(more )
Specimen
5D:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tectorial membrane |
Present |
|
5D
|
Expressed in the major and minor tectorial membranes.
|
Specimen
5E:
postnatal day 4
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 4
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5F:
postnatal day 6
(more )
Specimen
5F:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
5G:
postnatal day 8
(more )
Specimen
5G:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tectorial membrane |
Present |
|
5G
|
|
| TS28: cochlea epithelium |
Strong |
Regionally restricted |
5G
|
Expressed in Deiter's cells, especially in the third row of these cells that contribute to the formation of the marginal pillars.
|
Specimen
5H:
postnatal day 8
(more )
Specimen
5H:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 8
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tectorial membrane |
Present |
|
5H
|
|
| TS28: cochlea epithelium |
Strong |
Regionally restricted |
5H
|
Expressed in Deiter's cells, especially in the third row of these cells that contribute to the formation of the marginal pillars.
|
Specimen
5I:
postnatal day 20
(more )
Specimen
5I:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
postnatal day 20
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 17.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Rj:SWISS
|
|
Age:
|
embryonic day 17.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cochlear duct |
Present |
Regionally restricted |
|
Not expressed in the formed stria vascularis.
|
|
Analysis Tools