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Gene Expression Data
Assay Details
Reference: J:264256 Bernardo AS, et al., Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biol Open. 2018 Aug 17;7(8)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202678
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct-3/4 (H-134; sc-9081)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Specimen 5D mICM bottom: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Sema6a (assay MGI:6202663).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5D Oct4 mICM bottom , 5D Merge mICM bottom

Specimen 5D mERSE bottom: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Sema6a (assay MGI:6202663). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present 5D Oct4 mERSE bottom , 5D Merge mERSE bottom

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.17
The Jackson Laboratory