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Gene Expression Data
Assay Details
Assay
Reference: J:264256 Bernardo AS, et al., Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biol Open. 2018 Aug 17;7(8)
Assay type: RT-PCR
MGI Accession ID: MGI:6202667
Gene symbol: Gjb5
Gene name: gap junction protein, beta 5
Probe: Gjb5-pG, Gjb5-pH
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Expression was normalized against Gapdh.
Results
Image: 5C Gjb5 ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 163 bp (a) Amount Genetic BackgroundMutant Allele(s)Sex Note
ICM E3.5 TS4: inner cell mass Present 0.3 µg; total RNA (C57BL/6J x DBA/2J)F1 Not Specified ICM - Inner cell mass
Blast E3.5 TS4: blastocyst-stage conceptus Present 0.3 µg; total RNA (C57BL/6J x DBA/2J)F1 Not Specified Blast - Blastocyst
ERSE E6.25 TS8: epiblast Not Specified 0.3 µg; total RNA Not Specified Not Specified Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
APE E7.25 TS10: embryo Not Specified 0.3 µg; total RNA Not Specified Not Specified Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. APE - asymmetrical elongated anterior to posterior epiblast.
Notes:
(a) Expression was significantly higher in ICM stage embryos than in ERSE or APE stage embryos.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory