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Gene Expression Data
Assay Details
Reference: J:264256 Bernardo AS, et al., Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biol Open. 2018 Aug 17;7(8):bio033282
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202663
Gene symbol: Sema6a
Gene name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A
Antibody: Anti-Semaphorin 6A (AF1615)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Specimen 5D mICM: embryonic day 3.5 (more )
Note: Double labeled: green - Sema6a; red - Pou5f1 (assay MGI:6202678).
Structure Level Pattern Image Note
TS4: inner cell mass Weak 5D Merge mICM bottom , 5D Sema6a mICM

Specimen 5D mERSE: embryonic day 6.25 (more )
Note: Double labeled: green - Sema6a; red - Pou5f1 (assay MGI:6202678). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present 5D Merge mERSE bottom , 5D Sema6a mERSE

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.19
The Jackson Laboratory