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Gene Expression Data
Assay Details
Assay
Reference: J:264256 Bernardo AS, et al., Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biol Open. 2018 Aug 17;7(8)
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202660
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (N-19; sc-8628)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5D mICM top: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Gjb5 (assay MGI:6202661).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5D Oct4 mICM top , 5D Merge mICM top

Specimen 5D mICM middle: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Trip6 (assay MGI:6202662).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5D Oct4 mICM middle , 5D Merge mICM middle

Specimen 5D mERSE top: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Gjb5 (assay MGI:6202661). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present 5D Oct4 mERSE top , 5D Merge mERSE top

Specimen 5D mERSE middle: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Trip6 (assay MGI:6202662). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present 5D Merge mERSE middle , 5D Oct4 mERSE middle

Specimen S5F left: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Klf4 (assay MGI:6202677).
Structure Level Pattern Image Note
TS4: inner cell mass Present S5F Merge left , S5F Oct4 left

Specimen S5F right: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Klf4 (assay MGI:6202677). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present S5F Oct4 right , S5F Merge right


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory