Antibody:	anti-L3MBTL2-N
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS28: oocyte	Present		3A Oocyte	Expressed a rather uniform nuclear localization, but was excluded from the DAPI-rich regions.

Structure	Level	Pattern	Image	Note
TS1: 1-cell stage embryo	Present		3A Zygote	Present on both pronuclei.

Structure	Level	Pattern	Image	Note
TS2: 2-cell stage embryo	Weak		3A 2-cell	Significantly decreased expression.

Structure	Level	Pattern	Image	Note
TS3: 4-cell stage embryo	Weak		3A 4-cell	Gradually increased expression between the 4-cell and morula stages.

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Present		3A 8-cell	Gradually increased expression between the 4-cell and morula stages.

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Present		3A Morula	Gradually increased expression between the 4-cell and morula stages.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Present	Diffuse	3A Blastocyst	More diffuse signal, with high background.
TS4: inner cell mass	Weak		3A Blastocyst
TS4: trophectoderm	Weak		3A Blastocyst