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Gene Expression Data
Assay Details
Assay
Reference: J:59282 Yi SE, et al., The type I BMP receptor BMPRIB is required for chondrogenesis in the mouse limb. Development. 2000 Feb;127(3):621-30
Assay type: RNA in situ
MGI Accession ID: MGI:5806796
Gene symbol: Gdf5
Gene name: growth differentiation factor 5
Probe: Gdf5 cDNA7
Probe preparation: Antisense RNA
Results
Specimen 3c Wild type: embryonic day 12.0 (more )
Structure Level Pattern Image Note
TS20: hand Present Regionally restricted 3c wild type Expression was detected in regions of future joint formation.

Specimen 3c BmprIB-/-: embryonic day 12.0; Bmpr1btm1Kml/Bmpr1btm1Kml (more )
Structure Level Pattern Image Note
TS20: hand Present Regionally restricted 3c BmprIB-/- No difference was detected in the expression pattern in the mutant.

Specimen 3d Wild type: embryonic day 13.0 (more )
Structure Level Pattern Image Note
TS21: forelimb digit Present 3d wild type Expression was detected in the digital ray.

Specimen 3d BmprIB-/-: embryonic day 13.0; Bmpr1btm1Kml/Bmpr1btm1Kml (more )
Structure Level Pattern Image Note
TS21: forelimb digit Present 3d BmprIB-/- Expression in the digital ray was expanded in the mutant relative to the wild type pattern.

Specimen 3e Wild type: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: hand joint Present Regionally restricted 3e wild type Expression was restricted to the presumptive joint interzone and the cells that will form the synovial capsules.
TS21: forelimb interphalangeal joint Present Regionally restricted 3e wild type Expression was detected in the future synovial capsule of the interphalangeal joint.

Specimen 3e BmprIB-/-: embryonic day 13.5; Bmpr1btm1Kml/Bmpr1btm1Kml (more )
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 3e BmprIB-/- In the mutant, a single elongated domain of expression was observed in the region of the digital ray corresponding to the proximal and middle phalanges.
TS21: forelimb interphalangeal joint Weak Regionally restricted 3e BmprIB-/- Expression in the future synovial capsule of the interphalangeal joint is greatly reduced in the mutant.

Specimen 4c: embryonic day 13.5; Bmpr1btm1Kml/Bmpr1btm1Kml (more )
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 4c Expression was detected in the phalangeal region.

Specimen 4g Wild type: embryonic day 13.5 (more )
Note: The phalangeal region was defined by Gdf5 prior to detection of BrdU (alkaline phosphatase labeling).
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 4g wild type Expression was detected in the phalangeal region.

Specimen 4g BmprIB-/-: embryonic day 13.5; Bmpr1btm1Kml/Bmpr1btm1Kml (more )
Note: The phalangeal region was defined by Gdf5 prior to detection of BrdU (alkaline phosphatase labeling).
Structure Level Pattern Image Note
TS21: forelimb digit Present Regionally restricted 4g BmprIB-/- Expression was detected in the phalangeal region.

Specimen 5a,c: embryonic day 12.5 (more )
Note: Expression of Gdf5 (a) was shown in an overlay (c, green) on Bmpr1b expression (c, purple).
Structure Level Pattern Image Note
TS20: limb Present Regionally restricted 5c , 5a Gdf5 and Bmpr1b are expressed in nonoverlapping, but adjacent regions of the limb.

Specimen 5d,f: embryonic day 13.5 (more )
Note: Expression of Gdf5 (d) was shown in an overlay (f, green) on Bmpr1b expression (c, purple).
Structure Level Pattern Image Note
TS21: limb Present Regionally restricted 5d , 5f Gdf5 and Bmpr1b are expressed in nonoverlapping, but adjacent regions of the limb.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/05/2021
MGI 6.17
The Jackson Laboratory