Probe:	S100a1 cDNA
Probe preparation:	Antisense RNA labelled with S35

Structure	Level	Pattern	Image	Note
TS12: yolk sac	Very strong		6A
TS12: cardiac muscle tissue	Weak		6A

Structure	Level	Pattern	Image	Note
TS15: atrium cardiac muscle	Present		6B
TS15: primitive ventricle cardiac muscle	Present		6B

Structure	Level	Pattern	Image	Note
TS17: atrium cardiac muscle	Present		6C
TS17: heart septum	Present		6C
TS17: primitive ventricle cardiac muscle	Present		6C

Structure	Level	Pattern	Image	Note
TS19: heart septum	Present	Regionally restricted	6D	Expression is in the trabeculae.
TS19: ventricle cardiac muscle	Present	Regionally restricted	6D	Expression is in the trabeculae.

Structure	Level	Pattern	Image	Note
TS20: atrium cardiac muscle	Present		6E
TS20: ventricle cardiac muscle	Present		6E
TS20: endocardial cushion	Absent		6E
TS20: vena cava	Absent		6E

Structure	Level	Pattern	Image	Note
TS21: ventricle cardiac muscle	Present	Regionally restricted	6F	Expression is in the compact and trabecular layers of the ventricle.
TS21: atrium cardiac muscle	Weak		6F
TS21: atrioventricular valve	Absent		6F
TS21: endocardial cushion	Absent		6F

Structure	Level	Pattern	Image	Note
TS22: interventricular septum muscular part	Present		6G
TS22: ventricle cardiac muscle	Present	Regionally restricted	6G	Expression is in the compact and trabecular layers of the ventricle.
TS22: atrium cardiac muscle	Weak		6G

Structure	Level	Pattern	Image	Note
TS23: ventricle cardiac muscle	Present	Regionally restricted	6I	Expression is in the compact and trabecular layers of the ventricle.
TS23: atrium cardiac muscle	Weak		6I
TS23: aortic valve	Absent		6I	Absent in the non-muscle cells of the atrioventricular valve.
TS23: aorta	Absent		6I

Structure	Level	Pattern	Image	Note
TS24: ventricle cardiac muscle	Present	Regionally restricted	6J	Expression is in the compact and trabecular layers of the ventricle.
TS24: atrium cardiac muscle	Weak		6J
TS24: lung	Absent		6J
TS24: lung vascular element	Absent		6J

Structure	Level	Pattern	Image	Note
TS25: ventricle cardiac muscle	Strong	Regionally restricted	6K	Expression is in the compact and trabecular layers.
TS25: atrium cardiac muscle	Weak		6K
TS25: aorta	Absent		6K
TS25: pulmonary artery	Absent		6K