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Gene Expression Data
Assay Details
Assay
Reference: J:139734 Hafler BP, et al., Expression and function of Nkx6.3 in vertebrate hindbrain. Brain Res. 2008 Jul 30;1222:42-50
Assay type: RNA in situ
MGI Accession ID: MGI:5707643
Gene symbol: Nkx6-3
Gene name: NK6 homeobox 3
Probe: Nkx6-3 probe
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 5 Present 1A
TS17: rhombomere 6 Present 1A
TS17: rhombomere 7 Present 1A
TS17: rhombomere 8 Present 1A

Specimen 1C: embryonic day 10.5 (more )
Note: D is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Lhx3 (assay MGI:5707656).
Structure Level Pattern Image Note
TS17: rhombomere 7 Present Regionally restricted 1C , 1D Expression is in V2 neurons.

Specimen 1E: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1E Expression is in the V2a interneuron and motor neuron populations in the caudal hindbrain.

Specimen 1G: embryonic day 12.5 (more )
Note: H is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Vsx2 (assay MGI:5707659).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1G , 1H Expression is in the V2a interneuron and motor neuron populations in the caudal hindbrain.

Specimen 1I: embryonic day 12.5 (more )
Note: J is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Gata3 (assay MGI:5707666).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1I , 1J Expression is in the V2a interneuron and motor neuron populations in the caudal hindbrain.

Specimen 1K: embryonic day 12.5 (more )
Note: L is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Lhx3 (assay MGI:5707656).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 1K , 1L Expression is in the V2a/b interneuron and motor neuron populations in the caudal hindbrain.

Specimen 2A: embryonic day 12.5 (more )
Note: D is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Mnx1 (assay MGI:5707668).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 2D , 2A Expression is in postmitotic V2 interneurons and is absent in motor neurons in the caudal hindbrain.

Specimen 2B: embryonic day 12.5 (more )
Note: E is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Nkx6-1 (assay MGI:5707671).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 2E , 2B Expression is in postmitotic V2 interneurons in the caudal hindbrain.

Specimen 2C: embryonic day 12.5 (more )
Note: F is a confocal higher magnification image. Double labeled: red (pseudocolored) Nkx6-3; green - Mki67 (assay MGI:5707675).
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 2C , 2F Expression is in postmitotic V2 interneurons in the caudal hindbrain.

Specimen 2G: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 2G Expression is in V2a interneurons in the caudal hindbrain.

Specimen 2H: embryonic day 12.5; Olig2tm1Rth/Olig2tm1Rth (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted 2H The expression domain is expanded in this mutant.

Specimen S1 A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Regionally restricted S1A Expression is in the caudal hindbrain.

Specimen S1 B: embryonic day 12.5; Nkx6-3tm1Shiv/Nkx6-3tm1Shiv (more )
Structure Level Pattern Image Note
TS20: hindbrain Absent S1B


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/19/2021
MGI 6.17
The Jackson Laboratory