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Gene Expression Data
Assay Details
Assay
Reference: J:119509 Petrou P, et al., Overlapping and divergent localization of Frem1 and Fras1 and its functional implications during mouse embryonic development. Exp Cell Res. 2007 Mar 10;313(5):910-20
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5609957
Gene symbol: Frem1
Gene name: Fras1 related extracellular matrix protein 1
Antibody: Frem1 antibody
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 1A: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: surface ectoderm Present Regionally restricted 1A Authors report expression at the dermal-epidermal interface in the subepithelial matrix of the single-layered epidermis.

Specimen 1B: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: epidermis Present Regionally restricted 1B Expression at the subepithelial matrix of the two-layered epidermis.

Specimen 1C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: epidermis Present Regionally restricted 1C Expression at the subepithelial matrix of the three-layered epidermis.

Specimen 2A: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: epidermis Present Regionally restricted 2A Expression in the basement membrane and periderm cells in the outermost layer of the epidermis.

Specimen 2C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: epidermis Present Non-Uniform 2C Marked enhancement of expression in periderm cells. Expression is non-uniform and peaks at E16. Perinuclear localization of expression in basal keratinocytes.

Specimen 2E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: epidermis Present Regionally restricted 2E Expression in basement membrane, basal keratinocytes and keratinizing epidermal cells. Perinuclear localization of expression in basal keratinocytes. Expression in periderm no longer evident.

Specimen 3A: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: eyelid Present Regionally restricted 3A Expression in basement membrane of protruding eyeleids.
TS24: eyelid epithelium Present Regionally restricted 3A Expression in rounded periderm cells along the plane of fusion between the eyelids.

Specimen 3C: embryonic day 16.5; Fras1tm1Chpk/Fras1tm1Chpk (more )
Structure Level Pattern Image Note
TS24: eyelid Present Regionally restricted 3C Expression in basement membrane is completely abolished.
TS24: eyelid epithelium Present Regionally restricted 3C Expression in periderm cells is retained in the mutant embryo.

Specimen 4C: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: hair follicle Strong Regionally restricted 4C Prominent expression in hair follicle basement membrane and in interacting epithelial epidermal keratinocytes and condensing mesenchymal cells.

Specimen 4G: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: tooth Present Regionally restricted 4G Expression in basement membrane and interacting thickened epithelial cells and adjacent mesenchymal cells of bell-stage teeth.
TS23: tooth epithelium Present 4G
TS23: tooth mesenchyme Present 4G

Specimen 5B: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: lung epithelium Present Regionally restricted 5B Expression in basement membrane of respiratory epithelia.
TS22: visceral pleura Present 5B Expression in mesothelial layer of visceral pleura.

Specimen 5F: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: metanephros epithelium Present Regionally restricted 5F Expression in epithelial basememnt membranes.

Specimen 5J: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: intestine epithelium Present Regionally restricted 5J Expression in epithelial basement membranes.
TS22: gut mesentery Present Regionally restricted 5J Expression in intestinal mesothelium.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/28/2022
MGI 6.20
The Jackson Laboratory