Antibody:	anti-Frem1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Regionally restricted	1A	Authors report expression at the dermal-epidermal interface in the subepithelial matrix of the single-layered epidermis.

Structure	Level	Pattern	Image	Note
TS20: epidermis	Present	Regionally restricted	1B	Expression at the subepithelial matrix of the two-layered epidermis.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present	Regionally restricted	1C	Expression at the subepithelial matrix of the three-layered epidermis.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	2A	Expression in the basement membrane and periderm cells in the outermost layer of the epidermis.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Non-Uniform	2C	Marked enhancement of expression in periderm cells. Expression is non-uniform and peaks at E16. Perinuclear localization of expression in basal keratinocytes.

Structure	Level	Pattern	Image	Note
TS26: epidermis	Present	Regionally restricted	2E	Expression in basement membrane, basal keratinocytes and keratinizing epidermal cells. Perinuclear localization of expression in basal keratinocytes. Expression in periderm no longer evident.

Structure	Level	Pattern	Image	Note
TS24: eyelid	Present	Regionally restricted	3A	Expression in basement membrane of protruding eyeleids.
TS24: eyelid epithelium	Present	Regionally restricted	3A	Expression in rounded periderm cells along the plane of fusion between the eyelids.

Structure	Level	Pattern	Image	Note
TS24: eyelid	Present	Regionally restricted	3C	Expression in basement membrane is completely abolished.
TS24: eyelid epithelium	Present	Regionally restricted	3C	Expression in periderm cells is retained in the mutant embryo.

Structure	Level	Pattern	Image	Note
TS24: hair follicle	Strong	Regionally restricted	4C	Prominent expression in hair follicle basement membrane and in interacting epithelial epidermal keratinocytes and condensing mesenchymal cells.

Structure	Level	Pattern	Image	Note
TS23: tooth	Present	Regionally restricted	4G	Expression in basement membrane and interacting thickened epithelial cells and adjacent mesenchymal cells of bell-stage teeth.
TS23: tooth epithelium	Present		4G
TS23: tooth mesenchyme	Present		4G

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	5B	Expression in basement membrane of respiratory epithelia.
TS22: visceral pleura	Present		5B	Expression in mesothelial layer of visceral pleura.

Structure	Level	Pattern	Image	Note
TS22: metanephros epithelium	Present	Regionally restricted	5F	Expression in epithelial basememnt membranes.

Structure	Level	Pattern	Image	Note
TS22: intestine epithelium	Present	Regionally restricted	5J	Expression in epithelial basement membranes.
TS22: gut mesentery	Present	Regionally restricted	5J	Expression in intestinal mesothelium.