Antibody:	KIS antibody
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS11: allantois	Present	Regionally restricted	1A	Expression is in the outer cells of the allantois and some distal allantoic core cells.
TS11: amnion mesoderm	Present		1A
TS11: blood island	Present		1A
TS11: yolk sac mesoderm	Present		1A

Structure	Level	Pattern	Image	Note
TS12: allantois	Present	Regionally restricted	1B	Expression is in the outer cells of the allantois and some distal and basal allantoic core cells.
TS12: chorion ectoderm	Present		1B
TS12: chorion mesenchyme	Present		1B

Structure	Level	Pattern	Image	Note
TS13: chorion	Present	Regionally restricted	1C	Expression is in the mesothelium.

Structure	Level	Pattern	Image	Note
TS15: extraembryonic blood vessel	Present	Regionally restricted	1D	Expression is in the endothelium of the nascent allantoic vasculature.
TS15: allantois	Present	Regionally restricted	1D	Expression is in allantoic core cells.
TS15: extraembryonic component	Present	Regionally restricted	1D	Expression is in mesothelium.

Structure	Level	Pattern	Image	Note
TS15: chorioallantoic placenta	Strong	Regionally restricted	1E	Expression is in peripheral intermingled chorio-allantoic mesoderm.
TS15: allantois	Strong	Regionally restricted	1E	Expression is at the periphery of the chorio-allantoic fusion junction, where adhesion between allantoic and chorionic mesothelial cells secures the umbilicus onto the chorionic disk.
TS15: chorionic plate	Strong	Regionally restricted	1E	Expression is at the periphery of the chorio-allantoic fusion junction, where adhesion between allantoic and chorionic mesothelial cells secures the umbilicus onto the chorionic disk.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Present	Regionally restricted	1G	Expression is in syncytiotrophoblasts and in some trophoblast derivatives of the basal zone.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Present	Regionally restricted	1H	Expression is in syncytiotrophoblasts and in some trophoblast derivatives of the basal zone.

Structure	Level	Pattern	Image	Note
TS15: trophectoderm	Present	Regionally restricted	1I	Expression is in syncytiotrophoblasts.

Structure	Level	Pattern	Image	Note
TS11: chorion ectoderm	Present	Regionally restricted	2A
TS11: chorion mesoderm	Present	Regionally restricted	2A
TS11: ectoplacental cone	Weak		2A
TS11: primary trophoblast giant cell	Weak	Single cells	2A

Structure	Level	Pattern	Image	Note
TS13: chorion	Present	Regionally restricted	2B	Expression is in chorionic mesoderm and in the cytoplasm of peripheral chorionic ectoderm cells.

Structure	Level	Pattern	Image	Note
TS13: chorion	Present	Regionally restricted	2C	Expression is in the chorionic mesoderm and the apical edge of peripheral chorionic ectoderm cells.

Structure	Level	Pattern	Image	Note
TS13: chorion	Present	Regionally restricted	2D	Expression is in chorionic ectoderm.

Structure	Level	Pattern	Image	Note
TS11: trophectoderm	Present	Regionally restricted	2E	There is peripheral staining in secondary trophoblast giant cells.

Structure	Level	Pattern	Image	Note
TS13: extraembryonic component	Present	Single cells	2H , 2F	Expression is in some parietal endoderm cells.
TS13: trophoblast giant cell	Present		2H , 2F

Structure	Level	Pattern	Image	Note
TS28: decidua capsularis	Present		2F , 2H , 2I
TS28: decidua basalis	Weak		2F , 2K
TS28: decidua	Present	Regionally restricted	2J , 2F	Expression is in the decidua vascularis.

Structure	Level	Pattern	Image	Note
TS13: hindgut diverticulum endoderm	Present

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present	Regionally restricted	3A	Expression is in the anterior mesoderm.
TS11: node	Present		3A
TS11: primitive endoderm	Present		3A
TS11: neural ectoderm	Absent		3A

Structure	Level	Pattern	Image	Note
TS12: head mesenchyme	Present		3B
TS12: heart	Present		3B
TS12: neural ectoderm	Absent		3B
TS12: primitive streak	Absent		3B
TS12: somite	Absent		3B

Structure	Level	Pattern	Image	Note
TS13: embryo endoderm	Present		3C
TS13: heart	Present		3C
TS13: mesenchyme derived from head mesoderm	Present		3C
TS13: embryo	Present	Regionally restricted	3C	Expression is absent in the posterior region.
TS13: neural ectoderm	Absent		3C
TS13: somite	Absent		3C

Structure	Level	Pattern	Image	Note
TS13: head mesenchyme	Present		3D
TS13: heart	Present		3D

Structure	Level	Pattern	Image	Note
TS13: trunk	Present	Regionally restricted	3E	Expression is in the endodermal component of the trunk.
TS13: neural ectoderm	Present	Regionally restricted	3E	Expression is at the posterior surface of the trunk in surface ectoderm and/or neurectoderm.
TS13: surface ectoderm	Present	Regionally restricted	3E	Expression is at the posterior surface of the trunk in surface ectoderm and/or neurectoderm.
TS13: primitive streak	Absent		3E
TS13: somite	Absent		3E

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		3F
TS13: head mesenchyme	Present		3F
TS13: heart	Present		3F
TS13: surface ectoderm	Present	Regionally restricted	3F	Expression is in the surface ectoderm overlying the hindbrain.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	3G	Expression is in the dorsal tips prior to cranial fold closure.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	3H	Expression is in the dorsal tips during closure.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	3I	Expression is in the dorsal tips during closure.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present	Regionally restricted	3J	Expression is in the dorsal tips after closure.

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural plate	Present	Regionally restricted	4A	Expression is at the peripheral edges of the flattened neural plate.
TS14: surface ectoderm	Present	Regionally restricted	4A	Expression is in the surface ectoderm overlying the neural folds.
TS14: future spinal cord neural crest	Present		4A

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural fold	Present	Regionally restricted	4B	Expression is at the dorsal tips.
TS14: surface ectoderm	Present	Regionally restricted	4B	Expression is in the surface ectoderm overlying the neural folds.
TS14: future spinal cord neural crest	Present		4B

Structure	Level	Pattern	Image	Note
TS14: future spinal cord neural fold	Very strong	Regionally restricted	4C	Expression is at the dorsal tips.

Structure	Level	Pattern	Image	Note
TS14: neural tube	Very strong	Regionally restricted	4D	Expression is dorsal.
TS14: surface ectoderm	Present		4D

Structure	Level	Pattern	Image	Note
TS14: neural tube	Present	Regionally restricted	4E	Expression is dorsal and strong at the seam of closure.
TS14: notochord	Strong		4E
TS14: surface ectoderm	Present		4E	Expression is in the surface ectoderm overlying the neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4F	Expression is at the distal edges of the flat neural plate.
TS15: surface ectoderm	Present	Regionally restricted	4F	Expression is in the surface ectoderm overlying the neural folds.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		4G	Expression is at the distal edges of the closing neural plate.
TS15: surface ectoderm	Present	Regionally restricted	4G	Expression is in the surface ectoderm overlying the closing neural folds.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4H	Expression is at the distal edges of the closing neural plate.
TS15: surface ectoderm	Present	Regionally restricted	4H	Expression is in the surface ectoderm overlying the closing neural folds.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4I	Expression is at the distal edges of the closing neural plate.
TS15: surface ectoderm	Present	Regionally restricted	4I	Expression is in the surface ectoderm overlying the neural tube.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	4J	Expression is dorsal.
TS15: surface ectoderm	Present	Regionally restricted	4J	Expression is in the surface ectoderm overlying the neural tube.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4K	Expression is at the dorsal edges of the closing neural plate.
TS16: surface ectoderm	Present	Regionally restricted	4K	Expression is in the surface ectoderm overlying the neural folds.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4L	Expression is at the dorsal edges of the closing neural plate.
TS16: surface ectoderm	Present	Regionally restricted	4L	Expression is in the surface ectoderm overlying the closing neural folds.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4M	Expression is at the dorsal edges of the closing neural plate.
TS16: surface ectoderm	Present	Regionally restricted	4M	Expression is in the surface ectoderm overlying the closing neural folds.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4N	Expression is at the dorsal edges.
TS16: surface ectoderm	Present	Regionally restricted	4N	Expression is in the surface ectoderm overlying the neural tube.

Structure	Level	Pattern	Image	Note
TS16: neural tube	Present	Regionally restricted	4O	Expression is at the dorsal edges.
TS16: surface ectoderm	Present	Regionally restricted	4O	Expression is in the surface ectoderm overlying the neural tube.

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5A	Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5B	Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5C	Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5D	Expression is in the dorso-medial region of the closed neural tube, absent during secondary neurulation (shown).

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5E	Expression is in the dorso-medial region.

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5F	Expression is in the dorso-medial region.

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5G	Expression is in the dorso-medial region.

Structure	Level	Pattern	Image	Note
TS16: tail future spinal cord	Present	Regionally restricted	5H	Expression is in the dorso-medial region.