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Gene Expression Data
Assay Details
Assay
Reference: J:119482 Ohuchi H, et al., Diversified expression patterns of autotaxin, a gene for phospholipid-generating enzyme during mouse and chicken development. Dev Dyn. 2007 Mar 15;236(4):1134-1143
Assay type: RNA in situ
MGI Accession ID: MGI:5558035
Gene symbol: Enpp2
Gene name: ectonucleotide pyrophosphatase/phosphodiesterase 2
Probe: Atx cDNA1
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen 1A: embryonic day 8.5-8.75 (more )
Structure Level Pattern Image Note
TS13: neural fold Present Regionally restricted 1A Expression was detected in a narrow stripe in the cephalic neural folds.

Specimen 1A': embryonic day 8.5-8.75 (more )
Structure Level Pattern Image Note
TS13: neural fold Present Regionally restricted 1A' Expression was detected in the anterior neural folds posterior to the future closure of the neural tube at the forebrain-midbrain boundary.

Specimen 1B: embryonic day 8.5-8.75 (more )
Note: Two-color in situ: this assay, purple; Wnt1, red MGI:5558114.
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 1B The stripe of expression was almost overlapping with the posterior limit of Wnt1 expression in the midbrain.

Specimen 1C: embryonic day 8.75-9.0 (more )
Note: Two-color in situ: this assay, purple; Gbx2, red MGI:5558115.
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 1C Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was anterior to the Gbx2 expression domain of the hindbrain.

Specimen 1C": embryonic day 8.75-9.0 (more )
Note: Two-color in situ: this assay, purple; Gbx2, red MGI:5558115.
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 1C" Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was anterior to the Gbx2 expression domain of the hindbrain.

Specimen 1D: embryonic day 8.75-9.0 (more )
Note: Two-color in situ: this assay, purple; Pax2, red MGI:5559021.
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 1D Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was immediately anterior to the Pax2 expression domain of the midbrain-hindbrain boundary.

Specimen 1E: embryonic day 8.75-9.0 (more )
Note: One-color double in situ hybridization: see also Fgf8 MGI:5559023.
Structure Level Pattern Image Note
TS13: future midbrain Present Regionally restricted 1E Expression was detected in the midbrain abutting the midbrain-hindbrain boundary. Expression was immediately anterior to the Fgf8 expression domain of the midbrain-hindbrain boundary.

Specimen 1F: embryonic day 9.0-9.25 (more )
Structure Level Pattern Image Note
TS14: future midbrain Weak 1F Expression at the posterior end of the midbrain and in the vicinity of the midbrain-hindbrain boundary was weak.

Specimen 1H: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: 1st branchial arch Present 1H
TS15: branchial arch Present 1H
TS15: nasal process Present 1H
TS15: otocyst Present 1H
TS15: future midbrain Trace Regionally restricted 1H Expression was barely detected near the midbrain-hindbrain boundary.

Specimen 1I: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: otocyst Present 1I
TS15: future midbrain Trace Regionally restricted 1I Expression was barely detected near the midbrain-hindbrain boundary.

Specimen 1J: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: branchial arch Present 1J
TS17: latero-nasal process Present 1J
TS17: limb Present 1J
TS17: medial-nasal process Present 1J
TS17: neural tube Present 1J
TS17: hindbrain roof plate Present Regionally restricted 1J Expression was detected in the roof of the caudal hindbrain.

Specimen 1J': embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: neural tube floor plate Present 1J'

Specimen 1J": embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: branchial arch mesenchyme Present Regionally restricted 1J" Expression was detected in the subepithelial mesenchymal cells of the branchial arches.

Specimen 1K: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: nasal process Present 1K

Specimen 1K': embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: forelimb bud mesenchyme Present 1K'

Specimen 1L: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: hindbrain roof plate Present 1L

Specimen 1M: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere 1 Absent 1M Expression was not found in the upper rhombic lip region.

Specimen 1N: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere roof plate Present Regionally restricted 1N Expression was detected in the roof plate of the lower rhombic lip region.
TS17: hindbrain roof plate Present Regionally restricted 1N Expression was detected in the lower rhombic lip roof plate, but the midline roof plate cells of the hindbrain were devoid of expression.

Specimen 1O: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: rhombomere roof plate Present Regionally restricted 1O Expression was detected in the roof plate of the lower rhombic lip region.
TS17: hindbrain roof plate Present Regionally restricted 1O Expression was detected in the lower rhombic lip roof plate, but the midline roof plate cells of the hindbrain were devoid of expression.

Specimen 1P: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 1st branchial arch Present 1P
TS19: hindbrain Present 1P
TS19: limb Present 1P
TS19: maxillary process Present 1P
TS19: nasal process Present 1P
TS19: neural tube Present 1P
TS19: branchial arch Present 1P Expression was detected in the branchial region.

Specimen 1P inset: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: 1st branchial arch Present 1P inset Expression was detected in the branchial region.
TS19: branchial arch Present 1P inset Expression was detected in the branchial region.

Specimen 1Q: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: ear Present 1Q
TS20: limb Present 1Q
TS20: vibrissa Present 1Q
TS20: eye Present Regionally restricted 1Q Expression was detected in the eyelid canthus.
TS20: integumental system Present Regionally restricted 1Q Expression was detected in the mammary glands.

Specimen not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: intestine Present

Specimen 1R: embryonic day 12.5 (more )
Note: Embryo was midsagittally sliced and viewed from inside.
Structure Level Pattern Image Note
TS20: choroid plexus Present 1R
TS20: genital tubercle Present 1R
TS20: axial skeleton Present 1R Expression was detected in the vertebra.

Specimen 1S,U,V,W,X: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: 4th ventricle choroid plexus Present 1U , 1S
TS21: medulla oblongata ventricular layer Present 1U , 1S
TS21: pons ventricular layer Present 1U , 1S
TS21: embryo mesenchyme Present Regionally restricted 1V , 1S , 1W Expression was detected in the subepithelial mesenchyme of pharyngeal and cervical regions (V) and the subepithelial mesenchyme lining the embryo (W).
TS21: vibrissa dermal component Present Regionally restricted 1S , 1X Expression was detected in the dermal papilla of the vibrissae.

Specimen 1S inset: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: upper eyelid mesenchyme Present 1S inset

Specimen 1T: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: 4th ventricle choroid plexus Present 1T

Specimen 1Y: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: cochlear duct mesenchyme Present 1Y

Specimen 1Y': embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: thymus primordium Present 1Y'

Specimen 1Z: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: genital tubercle Present Regionally restricted 1Z Expression was detected in the subepithelial mesenchyme of the genital tubercle.

Specimen 1Z': embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: diaphragm Present 1Z'


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
06/23/2022
MGI 6.20
The Jackson Laboratory