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Gene Expression Data
Assay Details
Assay
Reference: J:171409 GUDMAP Consortium, GUDMAP: the GenitoUrinary Development Molecular Anatomy Project. www.gudmap.org. 2004;
Assay type: RNA in situ
MGI Accession ID: MGI:5540721
Gene symbol: Aff3
Gene name: AF4/FMR2 family, member 3
Probe: RIKEN clone A730046J16 subclone
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Results
Specimen GUDMAP:9653: embryonic day 10.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS17: genitourinary system Present Regionally restricted GUDMAP:9653
TS17: genitourinary system mesenchyme Present GUDMAP:9653
TS17: mesonephros Present Regionally restricted GUDMAP:9653
TS17: mesonephros mesenchyme Present GUDMAP:9653
TS17: nephric duct Absent GUDMAP:9653
TS17: nephric duct, metanephric portion Absent GUDMAP:9653
TS17: mesonephric tubule Ambiguous GUDMAP:9653 expression possible
TS17: metanephric mesenchyme Ambiguous GUDMAP:9653 most likely not there

Specimen GUDMAP:9654: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: male reproductive system Present Regionally restricted GUDMAP:9654
TS21: mesonephros of male Present Regionally restricted GUDMAP:9654
TS21: mesonephric mesenchyme of male Present GUDMAP:9654
TS21: testis Present Regionally restricted GUDMAP:9654
TS21: primary sex cord Present GUDMAP:9654
TS21: nephric duct of male, rest of Present GUDMAP:9654
TS21: mesenchyme of nephric duct of male Present GUDMAP:9654
TS21: paramesonephric duct of male, mesonephric portion Absent GUDMAP:9654
TS21: coelomic epithelium of male mesonephros Absent GUDMAP:9654
TS21: paramesonephric duct of male, rest of Absent GUDMAP:9654
TS21: epithelium of rest of paramesonephric duct of male Absent GUDMAP:9654
TS21: mesenchyme of rest of paramesonephric duct of male Absent GUDMAP:9654
TS21: mesonephric tubule of male Ambiguous GUDMAP:9654 expression likely in mesenchyme surrounding tubules
TS21: nephric duct of male, mesonephric portion Ambiguous GUDMAP:9654 possible expression in mesenchyme surrounding duct

Specimen GUDMAP:9655: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP. Male and female were examined; only the male is shown. Annotators reported the male expression pattern in the metanephros is identical to female.
Structure Level Pattern Image Note
TS21: urinary system Present Regionally restricted GUDMAP:9655
TS21: metanephros Present Regionally restricted GUDMAP:9655
TS21: nephrogenic interstitium Present GUDMAP:9655
TS21: renal interstitium Present GUDMAP:9655
TS21: ureter Present Regionally restricted GUDMAP:9655
TS21: ureteral mesenchyme Present GUDMAP:9655
TS21: cap mesenchyme Absent GUDMAP:9655
TS21: ureteric tip Absent GUDMAP:9655
TS21: renal capsule Ambiguous GUDMAP:9655 expression possible

Specimen GUDMAP:9656: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: female reproductive system Present Regionally restricted GUDMAP:9656
TS21: mesonephros of female Present Regionally restricted GUDMAP:9656
TS21: ovary Present Regionally restricted GUDMAP:9656
TS21: mesonephric mesenchyme of female Present GUDMAP:9656
TS21: nephric duct of female, rest of Present GUDMAP:9656
TS21: mesenchyme of nephric duct of female Present GUDMAP:9656
TS21: paramesonephric duct of female, mesonephric portion Absent GUDMAP:9656
TS21: mesonephric tubule of female Ambiguous GUDMAP:9656 expression likely in the mesenchyme surrounding the tubules
TS21: nephric duct of female, mesonephric portion Ambiguous GUDMAP:9656 possible expression in the mesenchyme surrounding the duct

Specimen GUDMAP:9657: embryonic day 13.5 (more )
Note: Labeling was visualized using NBT/BCIP.
Structure Level Pattern Image Note
TS21: urinary system Present Regionally restricted GUDMAP:9657
TS21: ureter Present Regionally restricted GUDMAP:9657
TS21: ureteral mesenchyme Present GUDMAP:9657
TS21: urogenital sinus Present GUDMAP:9657
TS21: primitive bladder Absent GUDMAP:9657

Specimen GUDMAP:9792: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple.
Structure Level Pattern Image Note
TS23: urinary system Present GUDMAP:9792
TS23: ureter Present GUDMAP:9792
TS23: ureteral mesenchyme Present GUDMAP:9792
TS23: ureteral smooth muscle layer Present GUDMAP:9792
TS23: metanephros Ambiguous GUDMAP:9792
TS23: renal cortex Ambiguous GUDMAP:9792
TS23: renal cortical interstitium Ambiguous GUDMAP:9792 possible weak expression
TS23: renal medulla Ambiguous GUDMAP:9792
TS23: renal medullary interstitium Ambiguous GUDMAP:9792 possible weak expression
TS23: renal pelvis Ambiguous GUDMAP:9792
TS23: pelvic smooth muscle Ambiguous GUDMAP:9792 possible weak expression
TS23: perihilar interstitium Ambiguous GUDMAP:9792 possible weak expression
TS23: ureteral mesenchyme outer layer Ambiguous GUDMAP:9792 possible weak expression
TS23: nephrogenic interstitium Ambiguous GUDMAP:9792 possible weak regional expression

Specimen GUDMAP:9793: embryonic day 15.5 (more )
Note: Labeling was visualized using BM purple.
Structure Level Pattern Image Note
TS23: ureter Present Regionally restricted GUDMAP:9793
TS23: ureteral mesenchyme Present GUDMAP:9793
TS23: ureteral smooth muscle layer Present GUDMAP:9793
TS23: urothelium of ureter Absent GUDMAP:9793
TS23: ureteral mesenchyme outer layer Absent GUDMAP:9793

Specimen GUDMAP:9794: postnatal adult (more )
Note: This animal was sexually mature. Labeling was visualized using BM purple.
Structure Level Pattern Image Note
TS28: metanephros Absent GUDMAP:9794
TS28: ureter Absent GUDMAP:9794

Specimen GUDMAP:9795: postnatal adult (more )
Note: This animal was sexually mature. Labeling was visualized using BM purple.
Structure Level Pattern Image Note
TS28: ureter Absent GUDMAP:9795


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/12/2019
MGI 6.14
The Jackson Laboratory