Gdf5 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:75699 Albrecht AN, et al., The synpolydactyly homolog (spdh) mutation in the mouse -- a defect in patterning and growth of limb cartilage elements. Mech Dev. 2002 Mar;112(1-2):53-67
Assay type:	RNA in situ
MGI Accession ID:	MGI:5446474
Gene symbol:	Gdf5
Gene name:	growth differentiation factor 5

Probe:	Gdf5 probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

Specimen 2 Gdf5 12.5 wt: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Regionally restricted	2_Gdf5 12.5 wt/wt	Expression in perichondrium of early digital anlage, with a striped pattern of expression in developing joints of digits.

Specimen 2 Gdf5 12.5 spdh/spdh: embryonic day 12.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS20: forelimb digit mesenchyme	Present	Regionally restricted	2_Gdf5 12.5 spdh/spdh	No expression in phalangeal anlagen.

Specimen 2 Gdf5 13.5 wt: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	2_Gdf5 13.5 wt/wt	Expression in developing joints of entire autopod.

Specimen 2 Gdf5 13.5 spdh/spdh: embryonic day 13.5; Hoxd13^spdh/Hoxd13^spdh (more

)

Structure	Level	Pattern	Image	Note
TS21: hand mesenchyme	Present	Regionally restricted	2_Gdf5 13.5 spdh/spdh	Some expression in joints between distal and middle phalanges, and between carpal bones. No expression in the joint interzone of digits.
TS21: forelimb digit 1 mesenchyme	Present	Regionally restricted	2_Gdf5 13.5 spdh/spdh	Some expression in metacarpophalangeal joint of digit 1,

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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