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Gene Expression Data
Assay Details
Assay
Reference: J:123586 Fitzgerald DP, et al., Neogenin is expressed on neurogenic and gliogenic progenitors in the embryonic and adult central nervous system. Gene Expr Patterns. 2007 Aug;7(7):784-92
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5440299
Gene symbol: Nes
Gene name: nestin
Antibody: Anti-Nestin
Detection system: Secondary antibody coupled to Alexa Fluor 568
Results
Specimen 1E/F: embryonic day 14.5 (more )
Note: Double labeled: red - Nes; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: hippocampus Present Regionally restricted 1F , 1E Expression was detected in neurogenic progenitors.
Specimen 1K/L: embryonic day 14.5 (more )
Note: Double labeled: red - Nes; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: olfactory cortex ventricular layer Present Regionally restricted 1L , 1K Expression was detected in neurogenic progenitors.
TS22: thalamus ventricular layer Present Regionally restricted 1L , 1K Expression was detected in neurogenic progenitors.
Specimen 2F: embryonic day 14.5 (more )
Note: Double labeled: red - Nes; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: midbrain ventricular layer Present Regionally restricted 2F Expression was detected in the radial glial processes in the dorsal midbrain ventricular zone.
TS22: midbrain Present Regionally restricted 2F Expression was detected in the radial glial processes spanning the more immature dorso-caudal midbrain.
Specimen 2H: embryonic day 14.5 (more )
Note: Double labeled: red - Nes; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: medulla oblongata Present Regionally restricted 2H Expression was detected in the radial glial processes spanning the medulla oblongata.
Specimen not shown: embryonic day 14.5 (more )
Note: Also stained for Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS22: cerebellum Present
Specimen 3E/F: embryonic day 17.5 (more )
Note: Double labeled: red - Nes; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS25: cerebral cortex subventricular zone Present Regionally restricted 3F , 3E Expression was detected in gliogenic precursors.
TS25: corpus striatum Present Regionally restricted 3F , 3E Expression was detected in gliogenic precursors.
TS25: hippocampus Present Regionally restricted 3F , 3E Expression was detected in gliogenic precursors.
Specimen 4D/E: postnatal week 9 (more )
Note: Double labeled: red - Nes; green - Neo1 (assay MGI:5440295). Very mild fixation in ethanol after cryosectioning.
Structure Level Pattern Image Note
TS28: lateral ventricle subependymal layer Present Regionally restricted 4D , 4E Expression was detected on the cell bodies and processes in the subventricular zone of the anterior corner of the lateral ventricle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory