Probe:	cdo cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: neural tube	Strong	Regionally restricted	2A	Expression is present in the dorsal neural tube.
TS15: dermomyotome	Strong		2A
TS15: future brain	Strong	Regionally restricted	2A	Expression is present in the dorsal aspects of the developing brain.

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present
TS17: pericardium	Present
TS17: endocardial lining	Absent

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	2B	Expression is present in the dorsal neural tube.
TS19: dermomyotome	Present		2B
TS19: forebrain	Present	Regionally restricted	2B	Expression is present in the dorsal forebrain.
TS19: midbrain	Present		2B
TS19: tongue	Present		2B
TS19: mandibular process	Present		2B
TS19: nasal process mesenchyme	Present		2B

Structure	Level	Pattern	Image	Note
TS21: forebrain ventricular layer	Present	Regionally restricted	2C	Expression is present in the dorsal forebrain.
TS21: midbrain ventricular layer	Present		2C
TS21: lung mesenchyme	Present	Regionally restricted	2C	Expression is present in the mesenchyme surrounding bronchi.
TS21: intestine	Present		2C
TS21: primitive bladder	Present		2C
TS21: head mesenchyme	Present	Regionally restricted	2C	Expression is present in the mesenchyme of facial structures.
TS21: upper lip	Present		2C
TS21: mandible mesenchyme	Present		2C
TS21: tongue	Present		2C
TS21: axial skeleton	Present	Regionally restricted	2C	Expression is present in the intervertebral disks.
TS21: trunk mesenchyme	Present	Regionally restricted	2C	Expression is present in the mesenchyme surrounding the ribs, intestines and bladder.
TS21: diaphragm	Present		2C
TS21: dermomyotome	Present		2C

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex ventricular layer	Present		2D
TS23: olfactory epithelium	Present		2D
TS23: skeletal muscle	Present		2D	Expression is present in muscle masses.
TS23: renal capsule	Present		2D
TS23: adrenal cortex	Present		2D
TS23: intervertebral disc	Present		2D
TS23: gut muscularis	Present		2D	Expression is present in the smooth muscle layers of the digestive tract (such as the esophagus, stomach and intestine).
TS23: vibrissa dermal component	Present		2D	Expression is present in the dermis of the whisker pad.
TS23: face mesenchyme	Present	Regionally restricted	2D	Expression is present in the mesenchyme of the whisker pad.
TS23: metanephros	Present	Non-Uniform	2D	Expression is present in the renal mesenchyme and tubules.
TS23: lung	Present		2D
TS23: tongue	Present		2D
TS23: trachea	Present		2D

Structure	Level	Pattern	Image	Note
TS23: pericardium	Present
TS23: endocardial tissue	Absent

Structure	Level	Pattern	Image	Note
TS25: intervertebral disc	Present		2E
TS25: gut muscularis	Present		2E	Expression is present in the smooth muscle layers of the digestive tract (such as the esophagus, stomach and intestine).
TS25: inner ear epithelium	Present		2E
TS25: vibrissa dermal component	Present		2E	Expression is present in the dermis of the whisker pad.
TS25: face mesenchyme	Present	Regionally restricted	2E	Expression is present in the mesenchyme of the whisker pad.
TS25: foot	Present		2E

Structure	Level	Pattern	Image	Note
TS25: reproductive system	Present	Regionally restricted		Expression is present in the mesenchyme and mesothelium of many reproductive tract structures.

Structure	Level	Pattern	Image	Note
TS25: mesorchium	Present	Regionally restricted		Expression is present in the mesenchyme and mesothelium of many reproductive tract structures.

Structure	Level	Pattern	Image	Note
TS25: oviduct	Present	Regionally restricted		Expression is present in the mesenchyme and mesothelium of many reproductive tract structures.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	3A/B	Expression is present in the dorsal neural tube.
TS15: somite	Strong	Regionally restricted	3A/B	Expression is present in the dorsolateral aspects of epithelialized somites. Lower levels of expression are seen in the cells of the myocele. There is no expression in the sclerotome.
TS15: trunk mesenchyme	Present	Regionally restricted	3A/B	Expression is present in the intermediate and body wall mesoderm and in the mesenchyme surrouding the hindgut diverticulum.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	3C/D	Expression is present in the dorsal lips.
TS17: dermomyotome	Strong		3C/D
TS17: sclerotome	Absent		3C/D
TS17: limb mesenchyme	Strong		3C/D	Expression is present in the proximal anterior region.
TS17: nephric duct, mesonephric portion	Present		3C/D	Expression is present in the mesonephric duct.
TS17: gonad primordium	Present		3C/D	Expression is present in the gonad primordium.

Structure	Level	Pattern	Image	Note
TS20: forelimb mesenchyme	Present		3E	Expression is present in the mesenchyme and premuscle masses.
TS20: forelimb pre-cartilage condensation	Absent		3E

Structure	Level	Pattern	Image	Note
TS23: forelimb musculature	Present		3F	Expression is present in the muscle masses.
TS23: forelimb dermis	Present		3F
TS23: forelimb epidermis	Absent		3F
TS23: forelimb pre-cartilage condensation	Absent		3F	There is no expression in the bones.

Structure	Level	Pattern	Image	Note
TS23: intervertebral disc	Present		3G
TS23: vertebra	Absent		3G
TS23: trachea mesenchyme	Present		3G
TS23: trachea cartilaginous ring	Absent		3G
TS23: trunk mesenchyme	Present	Regionally restricted	3G	Expression is present between the costal cartilage. There is no expression in the costal cartilage (the area where the ribs articulate with the vertebrae).

Structure	Level	Pattern	Image	Note
TS23: foot mesenchyme	Strong	Regionally restricted	3H	Expression is present in the mesenchyme surrounding the cartilage condensations and in the regions of joint formation (regions harboring proliferating cells of the epiphysial plate).
TS23: foot cartilage condensation	Absent		3H