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Gene Expression Data
Assay Details
Assay
Reference: J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type: RT-PCR
MGI Accession ID: MGI:5289533
Gene symbol: Adam23
Gene name: a disintegrin and metallopeptidase domain 23
Probe: Mm03053847_s1
Assay notes: These results were reported in Table S6. They were obtained using TaqMan real time PCR using individual blastomeres from either 2- or 4-cell embryos. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present, >=32.5 as absent, and 31.6-32.4 as ambiguous.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
2-cell-A1-1 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 1 from embryo A.
2-cell-A1-2 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 1 from embryo A.
2-cell-A2-1 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 2 from embryo A.
2-cell-A2-2 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 2 from embryo A.
2-cell-B1-1 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 1 from embryo B.
2-cell-B1-2 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 1 from embryo B.
2-cell-B2-1 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 2 from embryo B.
2-cell-B2-2 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 2 from embryo B.
2-cell-C1-1 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 1 from embryo C.
2-cell-C1-2 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 1 from embryo C.
2-cell-C2-1 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 2 from embryo C.
2-cell-C2-2 E1.5 TS2: 2-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 2 from embryo C.
4-cell-A1-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 1 from embryo A.
4-cell-A1-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 1 from embryo A.
4-cell-A2-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 2 from embryo A.
4-cell-A2-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 2 from embryo A.
4-cell-A3-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 3 from embryo A.
4-cell-A3-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 3 from embryo A.
4-cell-A4-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 4 from embryo A.
4-cell-A4-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 4 from embryo A.
4-cell-B1-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 1 from embryo B.
4-cell-B1-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 1 from embryo B.
4-cell-B2-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 2 from embryo B.
4-cell-B2-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 2 from embryo B.
4-cell-B3-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 3 from embryo B.
4-cell-B3-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 3 from embryo B.
4-cell-B4-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 4 from embryo B.
4-cell-B4-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 4 from embryo B.
4-cell-C1-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 1 from embryo C.
4-cell-C1-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 1 from embryo C.
4-cell-C2-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 2 from embryo C.
4-cell-C2-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 2 from embryo C.
4-cell-C3-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 3 from embryo C.
4-cell-C3-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 3 from embryo C.
4-cell-C4-1 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the first replicate using blastomere 4 from embryo C.
4-cell-C4-2 E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This is the second replicate using blastomere 4 from embryo C.
Notes:
(a) The Ct value of this reaction was 40.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/12/2019
MGI 6.14
The Jackson Laboratory