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Gene Expression Data
Assay Details
Assay
Reference: J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type: RT-PCR
MGI Accession ID: MGI:5289300
Gene symbol: Cdc23
Gene name: CDC23 cell division cycle 23
Probe: assay_14/19-FP_second allele, RP
Assay notes: These results were reported in Table S7. They were obtained using TaqMan real time PCR using individual blastomeres from 4-cell embryos. Two different primer sets were used with each sample in order to examine allele specific expression. The assay id presented in the raw CT values sheet of Table S7 differed from that associated with the primers in the assays design sheet; however, since results from only one assay were presented for this gene, it was assumed both ids referred to the same data. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present and >=32.5 as absent.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
4-cell-E1-B1-first allele Lane not used in this assay
4-cell-E1-B1-second allele E2.0 TS3: 4-cell stage embryo Absent (a) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 1.
4-cell-E1-B2-first allele Lane not used in this assay
4-cell-E1-B2-second allele E2.0 TS3: 4-cell stage embryo Absent (b) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 1.
4-cell-E2-B1-first allele Lane not used in this assay
4-cell-E2-B1-second allele E2.0 TS3: 4-cell stage embryo Present (c) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 2.
4-cell-E2-B2-first allele Lane not used in this assay
4-cell-E2-B2-second allele E2.0 TS3: 4-cell stage embryo Present (d) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 2.
4-cell-E3-B1-first allele Lane not used in this assay
4-cell-E3-B1-second allele E2.0 TS3: 4-cell stage embryo Absent (e) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 3.
4-cell-E3-B2-first allele Lane not used in this assay
4-cell-E3-B2-second allele E2.0 TS3: 4-cell stage embryo Absent (f) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 3.
Notes:
(a) The averaged Ct value from 3 technical replicates was 36.16093333.
(b) The averaged Ct value from 3 technical replicates was 34.780204.
(c) The averaged Ct value from 3 technical replicates was 25.20069133.
(d) The averaged Ct value from 3 technical replicates was 23.908295.
(e) The averaged Ct value from 3 technical replicates was 36.63293767.
(f) The averaged Ct value from 3 technical replicates was 36.66403333.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/12/2021
MGI 6.17
The Jackson Laboratory