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Gene Expression Data
Assay Details
Assay
Reference: J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type: RT-PCR
MGI Accession ID: MGI:5289277
Gene symbol: Nlrp4f
Gene name: NLR family, pyrin domain containing 4F
Probe: Nlrp4f-assay_6-FP_first allele, RP
Assay notes: These results were reported in Table S7. They were obtained using TaqMan real time PCR using individual blastomeres from 2-cell embryos. Two different primer sets were used with each sample in order to examine allele specific expression. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present and >=32.5 as absent.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
2-cell-E1-B1-first allele E1.5 TS2: 2-cell stage embryo Present (a) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 1.
2-cell-E1-B1-second allele Lane not used in this assay
2-cell-E1-B2-first allele E1.5 TS2: 2-cell stage embryo Present (b) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 1.
2-cell-E1-B2-second allele Lane not used in this assay
2-cell-E2-B1-first allele E1.5 TS2: 2-cell stage embryo Present (c) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 2.
2-cell-E2-B1-second allele Lane not used in this assay
2-cell-E2-B2-first allele E1.5 TS2: 2-cell stage embryo Present (d) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 2.
2-cell-E2-B2-second allele Lane not used in this assay
2-cell-E3-B1-first allele E1.5 TS2: 2-cell stage embryo Present (e) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 3.
2-cell-E3-B1-second allele Lane not used in this assay
2-cell-E3-B2-first allele E1.5 TS2: 2-cell stage embryo Present (f) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 3.
2-cell-E3-B2-second allele Lane not used in this assay
2-cell-E4-B1-first allele E1.5 TS2: 2-cell stage embryo Present (g) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 1 from embryo 4.
2-cell-E4-B1-second allele Lane not used in this assay
2-cell-E4-B2-first allele E1.5 TS2: 2-cell stage embryo Present (h) Not Specified; total RNA MF1 Not Specified This reaction used blastomere 2 from embryo 4.
2-cell-E4-B2-second allele Lane not used in this assay
Notes:
(a) The averaged Ct value from 3 technical replicates was 15.05015567.
(b) The averaged Ct value from 3 technical replicates was 15.362376.
(c) The averaged Ct value from 3 technical replicates was 13.82776533.
(d) The averaged Ct value from 3 technical replicates was 13.9586065.
(e) The averaged Ct value from 3 technical replicates was 13.64749533.
(f) The averaged Ct value from 3 technical replicates was 13.87025867.
(g) The averaged Ct value from 3 technical replicates was 14.057998.
(h) The averaged Ct value from 3 technical replicates was 14.39487267.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory