Antibody:	Anti-ShcD
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present		2D ShcD
TS22: heart	Present		2D ShcD
TS22: peripheral nervous system	Present		2D ShcD
TS22: skeleton	Present		2D ShcD

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Widespread	3_ShcD E12.5
TS20: brain	Present		3_ShcD E12.5
TS20: central nervous system	Present		3_ShcD E12.5
TS20: heart	Weak		3_ShcD E12.5

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present		3_ShcD E16.5
TS24: heart	Present		3_ShcD E16.5
TS24: liver	Present		3_ShcD E16.5
TS24: intestine mucosa	Present	Regionally restricted	3_ShcD E16.5	Expression was detected in cells of the mucosal layer.
TS24: intestine muscularis	Present	Regionally restricted	3_ShcD E16.5	Expression was detected in the longitudinal muscle wall of the intestine.
TS24: nucleus pulposus	Present	Regionally restricted	3_ShcD E16.5	Expression was detected in the hypertrophic chondrocytes and chondrocyte-like cells contained within the nucleus pulposus in the central region of the intervertebral discs.
TS24: skeleton	Present	Regionally restricted	3_ShcD E16.5	Expression was detected in populations of hypertrophic chondrocytes throughout the embryo.

Structure	Level	Pattern	Image	Note
TS24: liver	Present		3_ShcD Liver

Structure	Level	Pattern	Image	Note
TS24: intestine mucosa	Present	Regionally restricted	3_ShcD Gut	Expression was detected in cells of the mucosal layer.
TS24: intestine muscularis	Present	Regionally restricted	3_ShcD Gut	Expression was detected in the longitudinal muscle wall of the intestine.

Structure	Level	Pattern	Image	Note
TS24: nucleus pulposus	Present	Regionally restricted	3_ShcD Bone	Expression was detected in the hypertrophic chondrocytes and chondrocyte-like cells contained within the nucleus pulposus in the central region of the intervertebral discs.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present		3_ShcD Skin

Structure	Level	Pattern	Image	Note
TS24: heart	Present		3_ShcD Heart

Structure	Level	Pattern	Image	Note
TS15: heart	Present

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present
TS17: heart	Present
TS17: brain	Ambiguous			Table 1 indicates +/- expression in E10.5 brain.
TS17: surface ectoderm	Present			Expression was detected in skin.

Structure	Level	Pattern	Image	Note
TS19: brain	Present
TS19: dorsal root ganglion	Present
TS19: future spinal cord	Present
TS19: heart	Present
TS19: surface ectoderm	Present			Expression was detected in skin.

Structure	Level	Pattern	Image	Note
TS20: brain	Present
TS20: dorsal root ganglion	Present
TS20: heart	Present
TS20: skin	Present
TS20: spinal cord	Present

Structure	Level	Pattern	Image	Note
TS21: brain	Present
TS21: dorsal root ganglion	Present
TS21: heart	Present
TS21: skin	Present
TS21: spinal cord	Present
TS21: lung epithelium	Ambiguous			Table 1 indicates +/- expression in E13.5 lung epithelium.
TS21: metanephros	Ambiguous	Regionally restricted		Table 1 indicates +/- expression in E13.5 kidney epithelium.

Structure	Level	Pattern	Image	Note
TS22: adrenal gland	Present
TS22: brain	Present
TS22: cartilage	Present
TS22: dorsal root ganglion	Present
TS22: heart	Present
TS22: lung epithelium	Present
TS22: skin	Present
TS22: spinal cord	Present
TS22: tongue	Present
TS22: metanephros	Present	Regionally restricted		Expression was detected in kidney epithelium.
TS22: skeletal muscle	Present	Regionally restricted		Expression was detected in epaxial muscle.
TS22: cochlear duct epithelium	Ambiguous			Table 1 indicates +/- expression in E14.5 cochlear epithelium.

Structure	Level	Pattern	Image	Note
TS23: adrenal gland	Present
TS23: brain	Present
TS23: cartilage	Present
TS23: dorsal root ganglion	Present
TS23: eye	Present
TS23: gut	Present
TS23: heart	Present
TS23: lung epithelium	Present
TS23: skin	Present
TS23: spinal cord	Present
TS23: tongue	Present
TS23: vibrissa	Present
TS23: metanephros	Present	Regionally restricted		Expression was detected in kidney epithelium.
TS23: skeletal muscle	Present	Regionally restricted		Expression was detected in epaxial muscle.
TS23: cochlear duct epithelium	Present	Regionally restricted		Expression was detected in cochlear epithelium.
TS23: skeleton	Present	Regionally restricted		Expression was detected in intramembranous bone.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present	Regionally restricted	4A left	Expression was localized within neuronal tracts of the cerebral cortex.

Structure	Level	Pattern	Image	Note
TS22: dorsal root ganglion	Present		4A right , 4A middle

Structure	Level	Pattern	Image	Note
TS22: spinal cord marginal layer	Present	Regionally restricted	4B	Expression was localized primarily in neuronal fibers in the peripheral marginal layer or white matter.

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	4C	Expression was detected in bundles of axons projecting from the spinal column.

Structure	Level	Pattern	Image	Note
TS23: retina nerve fiber layer	Present		4D

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present	Regionally restricted	4E	Expression was localized within neuronal tracts of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Absent		4E	Expression was absent from the ventricular zone of the developing outer cortex (neopallial cortex).

Structure	Level	Pattern	Image	Note
TS22: medulla oblongata	Present	Regionally restricted	4F	Expression was localized within neuronal tracts of the medulla oblongata.

Structure	Level	Pattern	Image	Note
TS22: choroid plexus	Present	Regionally restricted	4G	Expression detected within the surface epithelial layer of the choroid plexus.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Present	Regionally restricted	5A	Expression was detected in the dorsal root ganglia and was particularly enriched in the nerve rootlets extending from the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS23: trigeminal ganglion	Present		5B
TS23: cochlear duct epithelium	Present		5B

Structure	Level	Pattern	Image	Note
TS22: vagus ganglion	Present		5C
TS22: vestibulo-cochlear ganglion	Present		5C

Structure	Level	Pattern	Image	Note
TS22: adrenal medulla	Present	Regionally restricted	5D	Expression was detected in the chromaffin cells of the adrenal medulla.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Regionally restricted		Expression was detected in the epidermis.

Structure	Level	Pattern	Image	Note
TS24: foot skin	Present		5E	Expression was prominent in the skin surrounding the footpad and toe.
TS24: hindlimb digit skin	Present		5E	Expression was prominent in the skin surrounding the footpad and toe.
TS24: foot epidermis	Present	Regionally restricted	5E	Expression was apparent in several layers of the stratified epidermis, but not the outermost cornified layer.

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present		5F	The section of skin was from the head. Expression in the surface epidermis was continuous with that seen in adjacent vibrissae follicles.
TS25: vibrissa epidermal component	Present	Regionally restricted	5F	In cross-section, expression appeared in a concentric ring characteristic of the outer epithelial root sheath and was limited in the surrounding dermal sheath of the follicles.
TS25: vibrissa dermal component	Weak	Regionally restricted	5F	In cross-section, expression appeared in a concentric ring characteristic of the outer epithelial root sheath and was limited in the surrounding dermal sheath of the follicles.

Structure	Level	Pattern	Image	Note
TS22: rib cartilage condensation	Present	Regionally restricted	6A	Expression was detected in the large cytoplasms of hypertrophic chondrocytes in ribs.
TS22: skeletal muscle	Present	Regionally restricted	6A	Expression was detected in the epaxial musculature of the trunk.

Structure	Level	Pattern	Image	Note
TS25: thoracic vertebra	Present	Regionally restricted	6B	Expression was detected in the large cytoplasms of hypertrophic chondrocytes in vertebral bodies.
TS25: epidermis	Strong	Regionally restricted	6B	Expression was strong in the basal and suprabasal layers of the epidermis.

Structure	Level	Pattern	Image	Note
TS25: fibula	Present	Regionally restricted	6C	Expression was restricted to the hypertrophic zone and absent in immature proliferating chondrocytes of long bones.
TS25: tibia	Present	Regionally restricted	6C	Expression was restricted to the hypertrophic zone and absent in immature proliferating chondrocytes of long bones.

Structure	Level	Pattern	Image	Note
TS24: basisphenoid bone	Present	Regionally restricted	6D	Expression was detected in the hypertrophic chondrocytes of the basisphenoid.

Structure	Level	Pattern	Image	Note
TS25: hyoid	Present	Regionally restricted	6E	Expression was detected in the hypertrophic chondrocytes of the developing hyoid.

Structure	Level	Pattern	Image	Note
TS24: Meckel's cartilage	Present	Regionally restricted	6F	Expression was detected in the hypertrophic chondrocytes of Meckel's cartilage.
TS24: lower jaw	Present	Regionally restricted	6F	Expression was detected in regions undergoing intramembranous ossification, such as the dentary that surrounds Meckel's cartilage.

Structure	Level	Pattern	Image	Note
TS15: outflow tract	Present			Expression was continuous between the outflow tract and primitive ventricle.
TS15: primitive ventricle	Present			Expression was continuous between the outflow tract and primitive ventricle.

Structure	Level	Pattern	Image	Note
TS20: heart atrium	Present	Widespread	7A
TS20: heart ventricle	Present	Widespread	7A
TS20: ventricle cardiac muscle	Present		7A
TS20: ventricle endocardial lining	Absent		7A
TS20: atrioventricular endocardial cushion	Present	Regionally restricted	7A	Expression was restricted in the atrio-ventricular cushion and was not expressed in the mesenchymal tissue that comprises the bulk of the atrio-ventricular cushions.

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Present		7B
TS24: tongue	Present		7B

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	7C	Expression was strong in the basal and suprabasal layers of the epidermis.
TS23: lens	Present		7C

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Present	Regionally restricted	7D	Expression was detected in the sheets of epithelial cells that line the lung bronchioles, particularly in the apical region of the cells.

Structure	Level	Pattern	Image	Note
TS25: renal tubule	Present	Regionally restricted	7E	Expression was detected in the sheets of epithelial cells that line the kidney tubules, particularly in the apical region of the cells.
TS25: glomerular tuft	Absent		7E	Expression could not be detected in visceral epithelial cells of the kidney glomerulus.

Structure	Level	Pattern	Image	Note
TS25: intestine muscularis	Present		7F	Expression was detected in the muscle layers of the intestinal wall.
TS25: intestine epithelium	Present		7F	Expression was detected in the sheets of epithelial cells that line the digestive tract, particularly in the apical region of the cells.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present		8A'

Structure	Level	Pattern	Image	Note
TS25: vibrissa follicle	Present	Regionally restricted	8B'	Expression was detected in the outer root sheath of the whisker follicles.

Structure	Level	Pattern	Image	Note
TS24: vertebra	Present	Regionally restricted	8C'	Expression was detected in chondrocytes of developing bone.