About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:165681 Hall JA, et al., Absence of thyroid hormone activation during development underlies a permanent defect in adaptive thermogenesis. Endocrinology. 2010 Sep;151(9):4573-82
Assay type: RT-PCR
MGI Accession ID: MGI:4881331
Gene symbol: Prkd1
Gene name: protein kinase D1
Probe: Prkd1-pA, Prkd1-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. Interscapular brown adipose tissue was used.
Results Image: 4A PKD1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
E16.5 E16.5 TS24: brown fat Present 0.3-1 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6J Not Specified
E17.5 E17.5 TS25: brown fat Present 0.3-1 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6J Not Specified
E18.5 E18.5 TS26: brown fat Present 0.3-1 µg; total RNA involves: 129S7/SvEvBrd * C57BL/6J Not Specified
E16.5 E16.5 TS24: brown fat Present 0.3-1 µg; total RNA B6.129S7-Dio2tm1Vag Dio2tm1Vag/Dio2+Not Specified
E17.5 E17.5 TS25: brown fat Present 0.3-1 µg; total RNA B6.129S7-Dio2tm1Vag Dio2tm1Vag/Dio2+Not Specified
E18.5 E18.5 TS26: brown fat Present 0.3-1 µg; total RNA B6.129S7-Dio2tm1Vag Dio2tm1Vag/Dio2+Not Specified
E16.5 E16.5 TS24: brown fat Present 0.3-1 µg; total RNA B6.129S7-Dio2tm1Vag Dio2tm1Vag/Dio2tm1VagNot Specified
E17.5 E17.5 TS25: brown fat Present 0.3-1 µg; total RNA B6.129S7-Dio2tm1Vag Dio2tm1Vag/Dio2tm1VagNot Specified
E18.5 E18.5 TS26: brown fat Present (a) 0.3-1 µg; total RNA B6.129S7-Dio2tm1Vag Dio2tm1Vag/Dio2tm1VagNot Specified
Notes:
(a) Expression was reduced 30% compared to wild-type at this age.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
10/05/2021
MGI 6.17
The Jackson Laboratory