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Gene Expression Data
Assay Details
Assay
Reference: J:158222 Demyanenko GP, et al., CHL1 cooperates with PAK1-3 to regulate morphological differentiation of embryonic cortical neurons. Neuroscience. 2010 Jan 13;165(1):107-15
Assay type: Immunohistochemistry
MGI Accession ID: MGI:4457154
Gene symbol: Pak2
Gene name: p21 (RAC1) activated kinase 2
Antibody: PAK1 (C19) antibody
Detection system: Secondary antibody coupled to fluorescein
Assay notes: This antibody recognizes Pak1-3.
Results
Specimen 1A: embryonic day 14.5 (more )
Note: vibratome section
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Strong 1A
TS22: cortical plate Strong 1A
TS22: cerebral cortex subventricular zone Present 1A
TS22: cerebral cortex ventricular layer Present 1A

Specimen 1B: embryonic day 15.5 (more )
Note: vibratome section
Structure Level Pattern Image Note
TS23: cerebral cortex Present Widespread 1B
TS23: cerebral cortex intermediate zone Strong Regionally restricted 1B Expression was high in axonal fibers.

Specimen 1C: embryonic day 15.5; Chl1tm1Mtg/Chl1tm1Mtg (more )
Note: vibratome section
Structure Level Pattern Image Note
TS23: cerebral cortex Present Widespread 1C Expression in this mutant is the same as in wild-type embryos.

Specimen 1D: embryonic day 17.5 (more )
Note: vibratome section
Structure Level Pattern Image Note
TS25: cerebral cortex intermediate zone Strong 1D
TS25: cerebral cortex ventricular zone Present 1D
TS25: cortical plate Present 1D

Specimen 1E: postnatal day 12 (more )
Note: vibratome section
Structure Level Pattern Image Note
TS28: cortical layer II Present 1E
TS28: cortical layer IV Present 1E
TS28: cortical layer III Present 1E The highest level of expression is in pyramidal cell soma and processes in layers III and V.
TS28: cortical layer V Present 1E The highest level of expression is in pyramidal cell soma and processes in layers III and V.
TS28: cortical layer VI Present 1E A somewhat lower level in layer VI.

Specimen 1J: embryonic day 15.5 (more )
Note: vibratome section. Double labeled: green - Pak2 (Pak1 MGI:4457146 and Pak3 MGI:4457155); red - Chl1 (assay MGI:4457151).
Structure Level Pattern Image Note
TS23: cerebral cortex intermediate zone Strong 1J PAK1-3 , 1J merge
TS23: cerebral cortex subventricular zone Present 1J merge , 1J PAK1-3

Specimen not shown: postnatal day 12; Chl1tm1Mtg/Chl1tm1Mtg (more )
Note: vibratome section
Structure Level Pattern Image Note
TS28: neocortex layer Present Expression in this mutant is the same as in wild-type in layers II-VI, with highest level of expression in pyramidal cell soma and processes in layers III and V, and somewhat lower levels in layer VI.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/12/2021
MGI 6.17
The Jackson Laboratory