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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450816
Gene symbol: Zfp445
Gene name: zinc finger protein 445
Probe: UI-M-BZ1-bkt-a-12-0-UI.s1
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Zfp445 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Strong Zfp445-Shh E11.5_coronal There is strong staining in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer Strong Zfp445-Shh E11.5_coronal There is strong staining in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Strong Zfp445-Shh E11.5_coronal There is strong staining in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Strong Zfp445-Shh E11.5_coronal There is strong staining in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Zfp445 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Moderate Zfp445-Shh E12.5_coronal There is moderate staining in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Zfp445 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Weak Zfp445-Shh E14.5_coronal There is weak staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Moderate Zfp445-Shh E14.5_coronal There is moderate staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate Weak Zfp445-Shh E14.5_coronal There is weak staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Weak Zfp445-Shh E14.5_coronal There is weak staining in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Weak Zfp445-Shh E14.5_coronal There is weak staining in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Moderate Non-Uniform Zfp445-Shh E14.5_coronal There is moderate staining in the ventricular zone of the ganglionic eminences and weak staining in the mantle zone.
TS22: hypothalamus mantle layer Weak Zfp445-Shh E14.5_coronal There is weak staining in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Weak Zfp445-Shh E14.5_coronal There is weak staining in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Weak Zfp445-Shh E14.5_coronal There is weak staining in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Weak Zfp445-Shh E14.5_coronal There is weak staining in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Zfp445 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Weak Zfp445-Shh E16.5_coronal There is weak staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Strong Zfp445-Shh E16.5_coronal There is strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate Weak Zfp445-Shh E16.5_coronal There is weak staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Weak Zfp445-Shh E16.5_coronal There is weak staining in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Weak Zfp445-Shh E16.5_coronal There is weak staining in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Weak Zfp445-Shh E16.5_coronal There is weak staining in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Weak Zfp445-Shh E16.5_coronal There is weak staining in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Weak Zfp445-Shh E16.5_coronal There is weak staining in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Weak Zfp445-Shh E16.5_coronal There is weak staining in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Weak Zfp445-Shh E16.5_coronal There is weak staining in the ventricular zone of the dorsal thalamus and prethalamus.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/12/2021
MGI 6.17
The Jackson Laboratory