About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450788
Gene symbol: Nap1l1
Gene name: nucleosome assembly protein 1-like 1
Probe: UI-M-BH3-auf-d-10-0-UI.s1
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Nap1l1 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Ambiguous Nap1l1-Shh E11.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer Moderate Nap1l1-Shh E11.5_coronal There is moderate staining in the ventricular zone of the dorsal thalamus, the prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: telencephalon mantle layer Ambiguous Nap1l1-Shh E11.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Moderate Nap1l1-Shh E11.5_coronal There is moderate staining in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Nap1l1 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Nap1l1-Shh E12.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Moderate Nap1l1-Shh E12.5_coronal There is moderate staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Nap1l1-Shh E12.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous Nap1l1-Shh E12.5_coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Moderate Nap1l1-Shh E12.5_coronal There is moderate staining in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Moderate Non-Uniform Nap1l1-Shh E12.5_coronal There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous Nap1l1-Shh E12.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Moderate Nap1l1-Shh E12.5_coronal There is moderate staining in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Ambiguous Nap1l1-Shh E12.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer Moderate Nap1l1-Shh E12.5_coronal There is moderate staining in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Nap1l1 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Moderate Nap1l1-Shh E14.5_coronal There is moderate staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS22: ganglionic eminence Moderate Non-Uniform Nap1l1-Shh E14.5_coronal There is moderate staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Weak Non-Uniform Nap1l1-Shh E14.5_coronal There is weak staining in the ventricular zone of the ventral POA and AVH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal POA, the PVH, VMH and ARH nuclei and anterior and posterior IDs.
TS22: thalamus mantle layer Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer Ambiguous Nap1l1-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Nap1l1 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Weak Nap1l1-Shh E16.5_coronal There is weak staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones.
TS24: ganglionic eminence Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous Nap1l1-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
01/14/2020
MGI 6.14
The Jackson Laboratory