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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450743
Gene symbol: Pitx2
Gene name: paired-like homeodomain transcription factor 2
Probe: MGC clone 96389
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Pitx2 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Very strong Non-Uniform Pitx2-Shh E11.5_sagittal , Pitx2-Shh E11.5_coronal Staining is very strong in the MZ of the mam & supramam zones & strong in prethalamus. Staining is either absent or ubiquitous in the MZ of the dorsal thalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, AVH, anterior & posterior IDs, premam zone & TT.
TS19: diencephalon lateral wall ventricular layer Very strong Non-Uniform Pitx2-Shh E11.5_sagittal , Pitx2-Shh E11.5_coronal There is very strong staining in the VZ of the mam & supramam zone. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, PVH, VMH & ARH nuclei, the AVH, anterior & posterior IDs, premam zone & TT.
TS19: telencephalon mantle layer Ambiguous Pitx2-Shh E11.5_sagittal , Pitx2-Shh E11.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Ambiguous Pitx2-Shh E11.5_sagittal , Pitx2-Shh E11.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Specimen Pitx2 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Ambiguous Pitx2-Shh E12.5_sagittal , Pitx2-Shh E12.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Ambiguous Pitx2-Shh E12.5_coronal , Pitx2-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate Ambiguous Pitx2-Shh E12.5_sagittal , Pitx2-Shh E12.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Very strong Non-Uniform Pitx2-Shh E12.5_coronal , Pitx2-Shh E12.5_sagittal There is very strong staining in the mantle zone of the mam and supramam zones. Staining is either absent or ubiquitous in the mantle zone of the premam zone and the TT.
TS20: diencephalon ventricular layer Very strong Non-Uniform Pitx2-Shh E12.5_coronal , Pitx2-Shh E12.5_sagittal There is very strong staining in the ventricular zone of the mam and supramam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam zone and the TT.
TS20: ganglionic eminence Ambiguous Pitx2-Shh E12.5_coronal , Pitx2-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous Pitx2-Shh E12.5_sagittal , Pitx2-Shh E12.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous Pitx2-Shh E12.5_coronal , Pitx2-Shh E12.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Very strong Non-Uniform Pitx2-Shh E12.5_sagittal , Pitx2-Shh E12.5_coronal There is very strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS20: thalamus ventricular layer Ambiguous Pitx2-Shh E12.5_sagittal , Pitx2-Shh E12.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Pitx2 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Ambiguous Pitx2-Shh E14.5_sagittal , Pitx2-Shh E14.5_coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Ambiguous Pitx2-Shh E14.5_sagittal , Pitx2-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate Ambiguous Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Very strong Non-Uniform Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal There is very strong staining in the mantle zone of the mam and supramam zones. Staining is either absent or ubiquitous in the mantle zone of the premam zone and the TT.
TS22: diencephalon ventricular layer Very strong Non-Uniform Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal There is very strong staining in the ventricular zone of the mam and supramam zones. Staining is either absent or ubiquitous in the ventricular zone of the premam zone and the TT.
TS22: ganglionic eminence Ambiguous Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Ambiguous Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: thalamus mantle layer Very strong Non-Uniform Pitx2-Shh E14.5_coronal , Pitx2-Shh E14.5_sagittal There is very strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS22: thalamus ventricular layer Ambiguous Pitx2-Shh E14.5_sagittal , Pitx2-Shh E14.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Pitx2 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous Pitx2-Shh E16.5_coronal , Pitx2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Ambiguous Pitx2-Shh E16.5_sagittal , Pitx2-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous Pitx2-Shh E16.5_coronal , Pitx2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Very strong Non-Uniform Pitx2-Shh E16.5_coronal , Pitx2-Shh E16.5_sagittal There is very strong staining in the mantle zone of the mam and supramam zones. Staining is either absent or ubiquitous in the mantle zone of the premam zone.
TS24: diencephalon ventricular layer Very strong Non-Uniform Pitx2-Shh E16.5_sagittal , Pitx2-Shh E16.5_coronal There is very strong staining in the ventricular zone of the mam and supramam zones. Staining is either absent or ubiquitous in the ventricular zone of the premam zone.
TS24: ganglionic eminence Ambiguous Pitx2-Shh E16.5_coronal , Pitx2-Shh E16.5_sagittal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous Pitx2-Shh E16.5_sagittal , Pitx2-Shh E16.5_coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Ambiguous Pitx2-Shh E16.5_sagittal , Pitx2-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: thalamus mantle layer Strong Non-Uniform Pitx2-Shh E16.5_coronal , Pitx2-Shh E16.5_sagittal There is strong staining in the mantle zone of the dorsal thalamus. Staining is either absent or ubiquitous in the mantle zone of the prethalamus.
TS24: thalamus ventricular layer Ambiguous Pitx2-Shh E16.5_sagittal , Pitx2-Shh E16.5_coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/19/2021
MGI 6.17
The Jackson Laboratory