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Gene Expression Data
Assay Details
Assay
Reference: J:157819 Shimogori T, et al., A genomic atlas of mouse hypothalamic development. Nat Neurosci. 2010;13(6):767-75
Assay type: RNA in situ
MGI Accession ID: MGI:4450742
Gene symbol: Ltbp1
Gene name: latent transforming growth factor beta binding protein 1
Probe: UI-M-AO1-aem-f-09-0-UI.s1
Probe preparation: Antisense RNA labelled with digoxigenin
Visualized with: Alkaline phosphatase
Assay notes: These specimens were also hybridized with a fluorescein-labeled Shh probe MGI:2110655; detected with TBT/BCIP. The results are reported in Supp. Table 4, whose legend includes a key to the abbreviations used and more fully details the regions examined.
Results
Specimen Ltbp1 - E11.5: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: diencephalon lateral wall mantle layer Weak Non-Uniform Ltbp1-Shh E11.5-coronal There is weak staining in the mantle zone of the dorsal thalamus and prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH nucleus, the AVH and anterior and posterior IDs.
TS19: diencephalon lateral wall ventricular layer Ambiguous Ltbp1-Shh E11.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, PVH nucleus, the AVH and anterior and posterior IDs.
TS19: telencephalon mantle layer Ambiguous Ltbp1-Shh E11.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer Weak Non-Uniform Ltbp1-Shh E11.5-coronal There is weak staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.

Specimen Ltbp1 - E12.5: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: cerebral cortex intermediate zone Moderate Ltbp1-Shh E12.5-coronal There is moderate staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer Moderate Ltbp1-Shh E12.5-coronal There is moderate staining in the ventricular zone of the cerebral cortex.
TS20: cortical plate Moderate Ltbp1-Shh E12.5-coronal There is moderate staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer Ambiguous Ltbp1-Shh E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer Ambiguous Ltbp1-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the premam, mam and supramam zones and the TT.
TS20: ganglionic eminence Ambiguous Ltbp1-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer Ambiguous Ltbp1-Shh E12.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer Ambiguous Ltbp1-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: thalamus mantle layer Strong Non-Uniform Ltbp1-Shh E12.5-coronal There is strong staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS20: thalamus ventricular layer Ambiguous Ltbp1-Shh E12.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Ltbp1 - E14.5: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cerebral cortex intermediate zone Moderate Scattered Ltbp1-Shh E14.5-coronal There is moderate, scattered staining in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer Very strong Ltbp1-Shh E14.5-coronal There is very strong staining in the ventricular zone of the cerebral cortex.
TS22: cortical plate Moderate Scattered Ltbp1-Shh E14.5-coronal There is moderate, scattered staining in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer Ambiguous Ltbp1-Shh E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam zone and the TT.
TS22: diencephalon ventricular layer Very strong Non-Uniform Ltbp1-Shh E14.5-coronal There is very strong staining in the ventricular zone of the premam zone. Staining is either absent or ubiquitous in the ventricular zone of the TT.
TS22: ganglionic eminence Strong Non-Uniform Ltbp1-Shh E14.5-coronal There is strong staining in the ventricular zone of the ganglionic eminences. Staining is either absent or ubiquitous in the mantle zone of the ganglionic eminences.
TS22: hypothalamus mantle layer Ambiguous Ltbp1-Shh E14.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer Very strong Non-Uniform Ltbp1-Shh E14.5-coronal There is very strong staining in the ventricular zone of the PVH, VMH and ARH nuclei and the AVH and strong staining in the dorsal POA. Staining is either absent or ubiquitous in the ventricular zone of the ventral POA and anterior and posterior IDs.
TS22: thalamus mantle layer Moderate Non-Uniform Ltbp1-Shh E14.5-coronal There is moderate staining in the mantle zone of the prethalamus. Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus.
TS22: thalamus ventricular layer Ambiguous Ltbp1-Shh E14.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Specimen Ltbp1 - E16.5: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cerebral cortex intermediate zone Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer Very strong Ltbp1-Shh E16.5-coronal There is very strong staining in the ventricular zone of the cerebral cortex.
TS24: cortical plate Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer Very strong Non-Uniform Ltbp1-Shh E16.5-coronal There is very strong staining in the ventricular zone of the premam and mam zones. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS24: ganglionic eminence Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer Very strong Non-Uniform Ltbp1-Shh E16.5-coronal There is very strong staining in the VZ of the PVH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH and moderate staining in the dorsal and ventral POAs. Staining is either absent or ubiquitous in the VZ of the SCH nucleus.
TS24: thalamus mantle layer Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer Ambiguous Ltbp1-Shh E16.5-coronal Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/04/2020
MGI 6.15
The Jackson Laboratory