Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:151330
Bai XH, et al., ADAMTS-7, a direct target of PTHrP, adversely regulates endochondral bone growth by associating with and inactivating GEP growth factor. Mol Cell Biol. 2009 Aug;29(15):4201-19
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:4365941
|
|
Gene symbol:
|
Adamts7
|
|
Gene name:
|
ADAM metallopeptidase with thrombospondin type 1 motif 7
|
|
Antibody:
|
anti-ADAMTS-7
|
|
Detection system:
|
Secondary antibody coupled to alkaline phosphatase
|
|
|
Results
|
Specimen
1Ca:
embryonic day 11.5
(more  )
Specimen
1Ca:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: embryo mesenchyme |
Present |
Regionally restricted |
1Ca
|
Expression in proliferating mesenchymal cells.
|
Specimen
1Cb:
embryonic day 13.5
(more )
Specimen
1Cb:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pre-cartilage condensation |
Present |
Regionally restricted |
1Cb
|
Expression clearly detectable in the center of a mesenchymal condensation and around it
|
Specimen
1Cc:
embryonic day 15.5
(more )
Specimen
1Cc:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 15.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cartilage |
Strong |
Regionally restricted |
1Cc
|
Prominent expression in proliferating and prehypertrophic chondrocytes.
|
Specimen
1Cd:
embryonic day 17.5
(more )
Specimen
1Cd:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cartilage |
Present |
Regionally restricted |
1Cd
|
Expression in proliferating and prehypertrophic chondrocytes in growth plates.
|
Specimen
1Ce:
postnatal newborn
(more )
Specimen
1Ce:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal newborn
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: long bone epiphyseal plate |
Present |
Regionally restricted |
1Ce
|
Expression in proliferating and prehypertrophic chondrocytes in growth plates.
|
Specimen
1Cf:
postnatal day 21
(more )
Specimen
1Cf:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 21
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
7Cb/c:
embryonic day 18.5
(more )
Specimen
7Cb/c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: bone |
Present |
Regionally restricted |
7Cc
,
7Cb
|
Expression in growth plate chondrocytes.
|
Specimen
7Ce/f:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas
|
|
Mutant Allele(s):
|
Pthlhtm1Hmk/Pthlhtm1Hmk
|
|
Age:
|
embryonic day 18.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: bone |
Weak |
Regionally restricted |
7Cf
,
7Ce
|
Expression in growth plate chondrocytes markedly reduced compared with wild type.
|
|
Analysis Tools