Probe:	Gek1 cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	5B , 5A	Expression is in spermatocytes in the late pachytene, diplotene, and diakinesis stages, and is absent in spermatpcytes at leptotene, zygotene, and early pachytene stages.
TS28: secondary spermatocyte	Present		5A , 5B
TS28: spermatid	Present	Regionally restricted	5B , 5A	Present in round spermatids, and absent in elongated spermatids.
TS28: spermatogonium	Absent		5B , 5A

Structure	Cell Type	Level	Pattern	Image	Note
TS21: germ cell of testis		Present		5C
TS21: interstitium of the testis		Present		5C	Expression is in Sertoli cells adjacent to positive germ cells and in some interstitial cells.
TS21: Sertoli cell	Sertoli cell	Present		5C	Expression is in Sertoli cells adjacent to positive germ cells and in some interstitial cells.

Structure	Level	Pattern	Image	Note
TS21: genital tubercle of male	Present		5E
TS21: mesonephros	Present		5E
TS21: metanephros	Present		5E
TS21: midbrain	Present		5E
TS21: spinal cord	Present		5E
TS21: telencephalon	Present		5E
TS21: head mesenchyme	Present	Regionally restricted	5E	Expression is in the facial mesenchyme.

Structure	Level	Pattern	Image	Note
TS28: oocyte	Trace			Little or no expression.
TS28: ovary stratum granulosum	Present