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Gene Expression Data
Assay Details
Assay
Reference: J:122362 Harwood BN, et al., Members of the WNT signaling pathways are widely expressed in mouse ovaries, oocytes, and cleavage stage embryos. Dev Dyn. 2008;237(4):1099-1111
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3775304
Gene symbol: Wnt7b
Gene name: wingless-type MMTV integration site family, member 7B
Modification date: 07/11/2008
Antibody: anti-Wnt-7a/b
Detection system: Secondary antibody coupled to Alexa Fluor 555
Assay notes: The antibody used in this assay recognizes both Wnt7a and Wnt7b. Analysis of staining patterns was done on at least two biological replicates.
Results
Specimen 3: postnatal day 21 (more )
Structure Level Pattern Image Note
TS28: meiotic oocyte Present 3 WNT7A/B The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression is likely to be due to the presence of Wnt7a protein.

Specimen 6--FGO: postnatal (more )
Structure Level Pattern Image Note
TS28: meiotic oocyte Present 6 WNT7A/B FGO The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression on the cell surface and in the cytoplasm of full grown oocytes is likely to be due to the presence of Wnt7a protein.

Specimen 6--OO: postnatal (more )
Structure Level Pattern Image Note
TS28: meiotic oocyte Present 6 WNT7A/B OO The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression on the cell surface and in the cytoplasm of ovulated oocytes is likely to be due to the presence of Wnt7a protein.

Specimen 6--Zyg: embryonic day 0.5 (more )
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Present 6 WNT7A/B Zyg The inability to amplify Wnt7b in RT-PCR reactions using material derived from this stage suggests that the observed expression on the cell surface and in the cytoplasm of zygotes is likely to be due to the presence of Wnt7a protein.

Specimen 6--2C: embryonic day 1.0 (more )
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 6 WNT7A/B 2C In 2-cell-stage embryos expression is primarily localized on the surface with some present in the cytoplasm.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/07/2017
MGI 6.11
The Jackson Laboratory