Ror2 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122362 Harwood BN, et al., Members of the WNT signaling pathways are widely expressed in mouse ovaries, oocytes, and cleavage stage embryos. Dev Dyn. 2008;237(4):1099-1111
Assay type:	RT-PCR
MGI Accession ID:	MGI:3774545
Gene symbol:	Ror2
Gene name:	receptor tyrosine kinase-like orphan receptor 2

Probe:	Ror2-N-pA, Ror2-N-pB
Visualized with:	Ethidium bromide
Assay notes:	The RT-PCR results are summarized in Fig. 2. Representative images of these results are presented in Supplemental Figure 1. Each reaction was performed more than once; results for each replicate are presented in Supplemental Tables 3 and 4.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
P0	P0	TS27: ovary	Present	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female
P6	P6	TS28: ovary	Present	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female
P8	P8	TS28: ovary	Present	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female
P12	P12	TS28: ovary	Present	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female
P21	P21	TS28: ovary	Present	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female
FGO	P	TS28: oocyte	Present	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female	Full-Grown Oocyte
OO	P	TS28: oocyte	Present (a)	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Female	Ovulated Oocyte
Zyg	E0.5 (b)	TS1: 1-cell stage conceptus	Present (a)	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Not Specified	Zygote
E2C	E1.0 (b)	TS2: 2-cell stage conceptus	Present (a)	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Not Specified	Early 2-Cell stage
L2C	E1.5 (b)	TS2: 2-cell stage conceptus	Present (a)	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Not Specified	Late 2-Cell stage
4-8C	E2.0 (b)	TS3: 4-8 cell stage conceptus	Absent	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Not Specified	4- to 8-Cell stage
Mor	E3.0 (b)	TS3: morula-stage conceptus	Absent	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Not Specified	Morula
Blast	E3.5 (b)	TS4: blastocyst-stage conceptus	Present (a)	Not Specified; total RNA	involves: C57BL/6J * DBA/2J		Not Specified	Blastocyst

Notes:
(a) The transcript was not present in all biological replicates; results for individual replicates are presented in Supplemental Tables 3 and 4.
(b) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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