Probe:	MH953 probe 838
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3B Csl
TS22: midbrain	Moderate		3B Csl
TS22: cerebellum	Strong		3B Csl
TS22: pons	Strong		3B Csl
TS22: medulla oblongata	Strong		3B Csl
TS22: spinal cord	Strong		3B Csl
TS22: cranial ganglion	Strong		3B Csl
TS22: dorsal root ganglion	Strong		3B Csl
TS22: olfactory epithelium	Moderate		3B Csl
TS22: intestine	Strong		3B Csl
TS22: salivary gland	Strong		3B Csl
TS22: tongue	Moderate		3B Csl
TS22: stomach	Moderate		3B Csl
TS22: liver	Strong		3B Csl
TS22: lung	Moderate		3B Csl
TS22: metanephros	Moderate		3B Csl
TS22: thyroid gland	Moderate		3B Csl
TS22: pituitary gland	Weak		3B Csl
TS22: pancreas	Moderate		3B Csl
TS22: adrenal gland	Weak		3B Csl
TS22: heart	Moderate		3B Csl
TS22: skeletal muscle	Moderate		3B Csl
TS22: cartilage	Absent		3B Csl	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3B Csl	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	MH953_3A , MH953_5A	There is broad expression throughout the embryo.
TS22: cranial ganglion	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: diencephalon	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: dorsal root ganglion	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: heart	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: intestine	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: liver	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: main olfactory bulb	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: medulla oblongata	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: metanephros	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: midbrain	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: neocortex	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: pons	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: salivary gland	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: skeletal muscle	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: spinal cord	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: thymus primordium	Present		MH953_3A , MH953_5A	This is one of the tissues with elevated expression levels.
TS22: axial skeleton	Absent		MH953_5A , MH953_3A
TS22: limb cartilage condensation	Absent		MH953_5A , MH953_3A	There is no expression in the long bones of the limb.