Probe:	MH1224 probe 1008
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3A Tpi
TS22: midbrain	Strong		3A Tpi
TS22: cerebellum	Strong		3A Tpi
TS22: pons	Strong		3A Tpi
TS22: medulla oblongata	Strong		3A Tpi
TS22: spinal cord	Strong		3A Tpi
TS22: cranial ganglion	Strong		3A Tpi
TS22: dorsal root ganglion	Strong		3A Tpi
TS22: olfactory epithelium	Strong		3A Tpi
TS22: intestine	Strong		3A Tpi
TS22: salivary gland	Strong		3A Tpi
TS22: tongue	Moderate		3A Tpi
TS22: stomach	Strong		3A Tpi
TS22: liver	Strong		3A Tpi
TS22: lung	Moderate		3A Tpi
TS22: metanephros	Moderate		3A Tpi
TS22: thyroid gland	Strong		3A Tpi
TS22: pituitary gland	Strong		3A Tpi
TS22: pancreas	Strong		3A Tpi
TS22: adrenal gland	Moderate		3A Tpi
TS22: heart	Strong		3A Tpi
TS22: skeletal muscle	Strong		3A Tpi
TS22: cartilage	Absent		3A Tpi	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Tpi	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	MH1224_4D	Expression is present throughout the embryo but its strength varies.
TS22: cerebral cortex ventricular layer	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: choroid plexus	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: cranial ganglion	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: heart	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: lens	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: liver	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: medulla oblongata	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: midbrain	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: pons	Present		MH1224_4D	This is one of the structures with an elevated expression level.
TS22: telencephalon	Present	Regionally restricted	MH1224_4D	There are scattered expressing cells located at the striatal-cortical interface.
TS22: spinal cord	Present	Non-Uniform	MH1224_4D	Expression is present in the spinal cord, most strongly in the motor column.
TS22: cerebellum	Present		MH1224_4D
TS22: intervertebral disc	Present		MH1224_4D
TS22: limb joint primordium	Present		MH1224_4D
TS22: lung	Present		MH1224_4D
TS22: peripheral nervous system	Present		MH1224_4D
TS22: piriform cortex	Present		MH1224_4D
TS22: pituitary gland	Present		MH1224_4D
TS22: retina	Present		MH1224_4D
TS22: skeletal muscle	Present		MH1224_4D
TS22: epidermis	Present		MH1224_4D	Expression is present in the epidermis. This includes the facial epithelium.