Probe:	MH2299 probe 1932
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Weak		3A Pfkp
TS22: midbrain	Moderate		3A Pfkp
TS22: cerebellum	Moderate		3A Pfkp
TS22: pons	Moderate		3A Pfkp
TS22: medulla oblongata	Moderate		3A Pfkp
TS22: spinal cord	Moderate		3A Pfkp
TS22: cranial ganglion	Moderate		3A Pfkp
TS22: dorsal root ganglion	Moderate		3A Pfkp
TS22: olfactory epithelium	Weak		3A Pfkp
TS22: intestine	Weak		3A Pfkp
TS22: salivary gland	Weak		3A Pfkp
TS22: tongue	Weak		3A Pfkp
TS22: stomach	Weak		3A Pfkp
TS22: liver	Weak		3A Pfkp
TS22: lung	Weak		3A Pfkp
TS22: metanephros	Weak		3A Pfkp
TS22: thyroid gland	Weak		3A Pfkp
TS22: pituitary gland	Weak		3A Pfkp
TS22: pancreas	Weak		3A Pfkp
TS22: adrenal gland	Weak		3A Pfkp
TS22: heart	Weak		3A Pfkp
TS22: skeletal muscle	Absent		3A Pfkp
TS22: cartilage	Absent		3A Pfkp	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Pfkp	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: central nervous system	Present		MH2299_3A	Expression is restricted to the central and peripheral nervous system.
TS22: peripheral nervous system	Present		MH2299_3A	Expression is restricted to the central and peripheral nervous system.
TS22: piriform cortex	Present		MH2299_3A
TS22: hypothalamus	Present	Regionally restricted	MH2299_3A	Expression is present in scattered neurons of the ventro-medial nucleus.
TS22: medulla oblongata	Present	Single cells	MH2299_3A	Expression is present in numerous single neurons.
TS22: midbrain	Present	Single cells	MH2299_3A	Expression is present in numerous single neurons.
TS22: pons	Present	Single cells	MH2299_3A	Expression is present in numerous single neurons.
TS22: thalamus	Present	Single cells	MH2299_3A	Expression is present in numerous single neurons.
TS22: cranial nerve	Strong		MH2299_3A	There is prominent expression in the cranial nerve nuclei.
TS22: dorsal root ganglion	Present		MH2299_3A
TS22: main olfactory bulb	Present		MH2299_3A
TS22: retina	Present		MH2299_3A
TS22: spinal cord mantle layer	Present	Regionally restricted	MH2299_3A	Expression is present in the motor neurons.