Probe:	MH1105 probe 942
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Strong		3A Gpi1
TS22: midbrain	Strong		3A Gpi1
TS22: cerebellum	Moderate		3A Gpi1
TS22: pons	Strong		3A Gpi1
TS22: medulla oblongata	Strong		3A Gpi1
TS22: spinal cord	Strong		3A Gpi1
TS22: cranial ganglion	Strong		3A Gpi1
TS22: dorsal root ganglion	Strong		3A Gpi1
TS22: olfactory epithelium	Strong		3A Gpi1
TS22: intestine	Strong		3A Gpi1
TS22: salivary gland	Strong		3A Gpi1
TS22: tongue	Moderate		3A Gpi1
TS22: stomach	Strong		3A Gpi1
TS22: liver	Strong		3A Gpi1
TS22: lung	Strong		3A Gpi1
TS22: metanephros	Moderate		3A Gpi1
TS22: thyroid gland	Moderate		3A Gpi1
TS22: pituitary gland	Strong		3A Gpi1
TS22: pancreas	Moderate		3A Gpi1
TS22: adrenal gland	Weak		3A Gpi1
TS22: heart	Strong		3A Gpi1
TS22: skeletal muscle	Moderate		3A Gpi1
TS22: cartilage	Absent		3A Gpi1	There is no expression in the axial cartilage.
TS22: skeleton	Absent		3A Gpi1	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Present	Non-Uniform	MH1105_3A	Expression is present in almost every part of the embryo and very strongly in some tissues.
TS22: cerebral cortex ventricular layer	Strong		MH1105_3A	There is strong expression in the ventricular zone of the developing neocortex.
TS22: spinal cord ventricular layer	Strong		MH1105_3A
TS22: spinal cord mantle layer	Strong	Regionally restricted	MH1105_3A	There is strong expression in the motor column along the spinal cord.
TS22: cranial ganglion	Strong		MH1105_3A
TS22: dorsal root ganglion	Strong		MH1105_3A
TS22: cranial nerve	Strong		MH1105_3A	There is strong expression in cranial nerve nuclei.
TS22: heart	Present		MH1105_3A
TS22: liver	Present		MH1105_3A
TS22: olfactory epithelium	Present		MH1105_3A
TS22: retina	Present		MH1105_3A
TS22: skeletal muscle	Present		MH1105_3A
TS22: vibrissa follicle	Present		MH1105_3A
TS22: pons	Present	Regionally restricted	MH1105_3A	Expresssion is present in the locus coeruleus.
TS22: midbrain	Present	Regionally restricted	MH1105_3A	Expression is present in many neurons.