Probe:	MH2298 probe 1931
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.

Structure	Level	Pattern	Image	Note
TS22: neocortex	Weak		3A Hk3
TS22: midbrain	Weak		3A Hk3
TS22: cerebellum	Weak		3A Hk3
TS22: pons	Weak		3A Hk3
TS22: medulla oblongata	Weak		3A Hk3
TS22: spinal cord	Weak		3A Hk3
TS22: cranial ganglion	Moderate		3A Hk3
TS22: dorsal root ganglion	Moderate		3A Hk3
TS22: olfactory epithelium	Weak		3A Hk3
TS22: intestine	Weak		3A Hk3
TS22: salivary gland	Weak		3A Hk3
TS22: tongue	Weak		3A Hk3
TS22: stomach	Weak		3A Hk3
TS22: liver	Weak		3A Hk3
TS22: lung	Weak		3A Hk3
TS22: metanephros	Weak		3A Hk3
TS22: thyroid gland	Weak		3A Hk3
TS22: pituitary gland	Weak		3A Hk3
TS22: pancreas	Weak		3A Hk3
TS22: adrenal gland	Weak		3A Hk3
TS22: heart	Weak		3A Hk3
TS22: skeletal muscle	Weak		3A Hk3
TS22: cartilage	Moderate	Regionally restricted	3A Hk3	Expression is present in the axial cartilage.
TS22: skeleton	Absent		3A Hk3	There is no expression in the long bones.

Structure	Level	Pattern	Image	Note
TS22: embryo	Weak		MH2298_3A	Expression is generally low and ubiquitous.
TS22: cranial ganglion	Strong	Single cells	MH2298_3A	There is strong expression in scattered neurons.
TS22: dorsal root ganglion	Strong	Single cells	MH2298_3A	There is strong expression in scattered neurons.
TS22: cartilage	Present		MH2298_3A	Expression is markedly elevated in the perichondrium of the axial skeleton.