Gene Expression Data
Assay Details
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Assay
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Reference:
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J:101025
Yaylaoglu MB, et al., Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform. Dev Dyn. 2005 Oct;234(2):371-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3766942
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Gene symbol:
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Fgf17
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Gene name:
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fibroblast growth factor 17
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Probe:
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FG51 probe 16
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.
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Results
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Specimen
4G:
embryonic day 14.5
(more  )
Specimen
4G:
(close  )
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Genetic Background:
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either: C57BL/6J or NMRI
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindbrain |
Present |
Regionally restricted |
4G
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Expression is present at the midbrain-hindbrain boundary.
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| TS22: midbrain |
Present |
Regionally restricted |
4G
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Expression is present at the midbrain-hindbrain boundary.
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Specimen
FG51:
embryonic day 14.5
(more )
Specimen
FG51:
(close )
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Genetic Background:
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either: C57BL/6J or NMRI
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: hindbrain |
Present |
Regionally restricted |
FG51
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Expression is restricted to the midbrain/hindbrain junction.
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| TS22: midbrain |
Present |
Regionally restricted |
FG51
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Expression is restricted to the midbrain/hindbrain junction.
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| TS22: forebrain |
Present |
Regionally restricted |
FG51
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Expression is restricted to the lamina terminalis.
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| TS22: telencephalon septum |
Present |
Regionally restricted |
FG51
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Expression is restricted to subfornical organ.
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| TS22: hypothalamus |
Present |
Regionally restricted |
FG51
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Expression is present in a very restricted way in the suprachiasmatic region.
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Analysis Tools