Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:101025
Yaylaoglu MB, et al., Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform. Dev Dyn. 2005 Oct;234(2):371-86
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3766941
|
|
Gene symbol:
|
Fgf10
|
|
Gene name:
|
fibroblast growth factor 10
|
|
Probe:
|
MH359 probe 302
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection. Authors did not specify which probe was used to obtain these results; it is possible MGI:3724327 was used instead.
|
|
|
Results
|
Specimen
4F:
embryonic day 14.5
(more  )
Specimen
4F:
(close  )
|
Genetic Background:
|
either: C57BL/6J or NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
Specimen
FG47, MH359:
embryonic day 14.5
(more )
Specimen
FG47, MH359:
(close )
|
Genetic Background:
|
either: C57BL/6J or NMRI
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
Fresh Frozen
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: facial ganglion |
Present |
|
MH359
,
FG47
|
|
| TS22: trigeminal ganglion |
Present |
|
MH359
,
FG47
|
|
| TS22: pons |
Present |
Regionally restricted |
FG47
,
MH359
|
Expression is present in the superior paraolivary nucleus.
|
| TS22: medulla oblongata |
Present |
Regionally restricted |
MH359
,
FG47
|
Expression is present in the ambiguous nucleus.
|
| TS22: pituitary gland |
Present |
Regionally restricted |
FG47
,
MH359
|
Expression is present in the posterior part of the pituitary anlage.
|
| TS22: hypothalamus ventricular layer |
Present |
|
MH359
,
FG47
|
Expression is present in the hypothalamic neuroepithelium.
|
| TS22: spinal cord |
Present |
|
FG47
,
MH359
|
|
| TS22: ear |
Strong |
|
MH359
,
FG47
|
|
| TS22: lung mesenchyme |
Strong |
|
MH359
,
FG47
|
|
| TS22: perioptic mesenchyme |
Strong |
|
MH359
,
FG47
|
|
| TS22: submandibular gland primordium mesenchyme |
Strong |
|
MH359
,
FG47
|
|
| TS22: gland |
Strong |
Regionally restricted |
MH359
,
FG47
|
Expression is present in lacrimal gland tissue.
|
| TS22: lower jaw incisor dental papilla |
Strong |
|
MH359
,
FG47
|
|
| TS22: upper jaw incisor dental papilla |
Strong |
|
MH359
,
FG47
|
|
| TS22: intestine |
Present |
|
MH359
,
FG47
|
|
| TS22: metanephros |
Present |
|
MH359
,
FG47
|
|
| TS22: tongue |
Present |
|
MH359
,
FG47
|
|
| TS22: trunk mesenchyme |
Present |
Regionally restricted |
MH359
,
FG47
|
Expression is present in tissue around the thymus.
|
| TS22: sternum |
Present |
Regionally restricted |
MH359
,
FG47
|
Expression is present in the rostral part of the sternum.
|
| TS22: appendicular skeleton |
Present |
Regionally restricted |
FG47
,
MH359
|
Expression is present in the perichondrium of the distal-most limb skeletal elements.
|
| TS22: forearm dermis |
Present |
|
MH359
,
FG47
|
|
| TS22: lower leg dermis |
Present |
|
MH359
,
FG47
|
|
|
Analysis Tools