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Gene Expression Data
Assay Details
Assay
Reference: J:101025 Yaylaoglu MB, et al., Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform. Dev Dyn. 2005 Oct;234(2):371-86
Assay type: RNA in situ
MGI Accession ID: MGI:3766934
Gene symbol: Fgf2
Gene name: fibroblast growth factor 2
Probe: FG39 probe 25
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.
Results
Specimen 4K: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: radius cartilage condensation Weak 4K
TS22: ulna cartilage condensation Weak 4K
Specimen FG39: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: central nervous system Trace FG39
TS22: brain Present FG39 At high magnification, tiny blue dye precipitates are detected throughout the brain.
TS22: embryo Weak FG39 Expression is possibly ubiquitous.
TS22: appendicular skeleton Present FG39 Expression is present in the long bones of the developing limb.
TS22: cartilage Present Regionally restricted FG39 Expression is present in the facial and axial cartilage.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
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