Gene Expression Data
Assay Details
Assay
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Reference:
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J:117221
Shibukawa Y, et al., Temporomandibular joint formation and condyle growth require Indian hedgehog signaling. Dev Dyn. 2007 Feb;236(2):426-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3713086
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Gene symbol:
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Sox9
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Gene name:
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SRY (sex determining region Y)-box 9
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Probe:
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Sox9 cDNA13
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Assay notes:
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Following photography,
gene expression patterns were given computer-generated artificial colors and viewed along or
superimposed. Sox9 is shown in red.
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Results
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Specimen
4A:
embryonic day 15.5
(more )
Note: Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
Specimen
4A:
(close )
Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mandible |
Strong |
Regionally restricted |
4A
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Expression is in the polymorphic layer and in the flattened chondrocyte zone of the condyle.
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Specimen
4E:
(close )
Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mandible |
Weak |
Regionally restricted |
4E
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Expression is in the polymorphic layer and in the flattened chondrocyte zone of the condyle.
|
Specimen
4I:
(close )
Genetic Background:
|
involves: 129 * C3H/HeJ * C57BL/6J
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Mutant Allele(s):
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Gli3Xt-J/Gli3Xt-J Ihhtm1Amc/Ihhtm1Amc
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Age:
|
embryonic day 15.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: mandible |
Present |
Regionally restricted |
4I
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Expression is reminiscent of, albeit not identical to, that in wild-type.
|
Specimen
4M:
embryonic day 18.5
(more )
Note: Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
Specimen
4M:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Strong |
Regionally restricted |
4M
|
Expression is in the polymorphic layer and in the flattened chondrocyte zone of the condyle.
|
Specimen
4Q:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
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Mutant Allele(s):
|
Ihhtm1Amc/Ihhtm1Amc
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Weak |
Regionally restricted |
4Q
|
Expression is in the polymorphic layer and in the flattened chondrocyte zone of the condyle.
|
Specimen
4U:
(close )
Genetic Background:
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involves: 129 * C3H/HeJ * C57BL/6J
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Mutant Allele(s):
|
Gli3Xt-J/Gli3Xt-J Ihhtm1Amc/Ihhtm1Amc
|
Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Sox9; green - Col2a1 (assay MGI:3713091).
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Present |
Regionally restricted |
4U
|
Expression is reminiscent of, albeit not identical to, that in wild-type.
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