About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:107770 Nakamura K, et al., Endogenous and exogenous glucocorticoid regulation of ENaC mRNA expression in developing kidney and lung. Am J Physiol Cell Physiol. 2002 Sep;283(3):C762-72
Assay type: RNase protection
MGI Accession ID: MGI:3625187
Gene symbol: Scnn1g
Gene name: sodium channel, nonvoltage-gated 1 gamma
Probe: gamma-mENaC cDNA1
Probe preparation: Antisense RNA labelled with biotin
Visualized with: Autoradiography
Assay notes: Shown is a graph of the quantitative analysis. Relative abundance of mENaC for CRH knock-out and wild-type mice normalized to 18S RNA and to the abundance at E18.5.
Results Image: 2_gamma kidney
 Sample Information Bands Other Sample Information
Lane AgeStructure 191 b (a) Amount Genetic BackgroundMutant Allele(s)Sex
wt Kidney E16.5 TS24: metanephros Present 25 µg; total RNA Not Specified Not Specified
wt Kidney E17.5 TS25: metanephros Present 25 µg; total RNA Not Specified Not Specified
wt Kidney E18.5 TS26: metanephros Present 25 µg; total RNA Not Specified Not Specified
CRH-KO Kidney E16.5 TS24: metanephros Present 25 µg; total RNA involves: 129S2/SvPas * C57BL/6J Crhtm1Maj/Crhtm1MajNot Specified
CRH-KO Kidney E17.5 TS25: metanephros Present 25 µg; total RNA involves: 129S2/SvPas * C57BL/6J Crhtm1Maj/Crhtm1MajNot Specified
CRH-KO Kidney E18.5 TS26: metanephros Present 25 µg; total RNA involves: 129S2/SvPas * C57BL/6J Crhtm1Maj/Crhtm1MajNot Specified
Notes:
(a) In both mutant and wild-type embryos there is an increase in expression with increasing age.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
10/19/2021
MGI 6.17
The Jackson Laboratory