Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:103446
McKee AE, et al., A genome-wide in situ hybridization map of RNA-binding proteins reveals anatomically restricted expression in the developing mouse brain. BMC Dev Biol. 2005 Jul 20;5:14
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3617754
|
|
Gene symbol:
|
Spen
|
|
Gene name:
|
spen family transcription repressor
|
|
Probe:
|
Mint cDNA1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
The results of this assay were listed in Additional File 3, row Mint.
|
|
|
Results
|
Specimen
E13.5:
embryonic day 13.5
(more  )
Note: Authors indicate that there was restricted expression in the nervous system with distinguishable expression in non-neural tissue.
Specimen
E13.5:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Authors indicate that there was restricted expression in the nervous system with distinguishable expression in non-neural tissue.
|
Specimen
P0:
postnatal day 0
(more )
Note: Authors indicate that there was restricted expression in the nervous system with distinguishable expression in non-neural tissue.
Specimen
P0:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
postnatal day 0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Note:
|
Authors indicate that there was restricted expression in the nervous system with distinguishable expression in non-neural tissue.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cerebral cortex |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: corpus striatum |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: hindbrain |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: hippocampus |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: hypothalamus |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: main olfactory bulb |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: midbrain |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: olfactory epithelium |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: retina |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: spinal cord |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: thalamus |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: thymus |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: tooth |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
| TS27: trigeminal ganglion |
Present |
|
R0272-23
,
R0272-22
,
R0272-21
|
|
|
Analysis Tools