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Gene Expression Data
Assay Details
Assay
Reference: J:45489 Zabludoff SD, et al., p27Kip1 is expressed transiently in developing myotomes and enhances myogenesis. Cell Growth Differ. 1998 Jan;9(1):1-11
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3611792
Gene symbol: Cdkn1b
Gene name: cyclin-dependent kinase inhibitor 1B
Antibody: Anti-p27
Detection system: Secondary antibody coupled to fluorescein
Results
Specimen not shown: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: trunk myotome Present Regionally restricted Expression was detected in only a few cells of forelimb level myotomes but no other cells in the paraxial mesoderm.
TS15: trunk paraxial mesenchyme Present Regionally restricted Expression was detected in only a few cells of forelimb level myotomes but no other cells in the paraxial mesoderm.

Specimen 1A,1D: embryonic day 10.5 (more )
Note: This specimen was coimmunostained with Myogenin (see Assay MGI:3611793).
Structure Level Pattern Image Note
TS17: trunk myotome Present Regionally restricted 1A , 1D Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.
TS17: trunk paraxial mesenchyme Present Regionally restricted 1A , 1D Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.

Specimen 2A p27: embryonic day 10.5 (more )
Note: This specimen was coimmunostained with MyoD (see Assay MGI:3611794).
Structure Level Pattern Image Note
TS17: trunk myotome Present Regionally restricted 2A p27 , 2A MyoD/p27 Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. All cells that were immunopositive were also positive for the expression of MyoD (see Assay MGI:3611794).
TS17: trunk paraxial mesenchyme Present Regionally restricted 2A p27 , 2A MyoD/p27 Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. All cells that were immunopositive were also positive for the expression of MyoD (see Assay MGI:3611794).

Specimen 2B p27: embryonic day 10.5 (more )
Note: This specimen was coimmunostained with [alpha]-Actinin (see Assays MGI:3611795 & MGI:3611796).
Structure Level Pattern Image Note
TS17: trunk myotome Present Regionally restricted 2B [alpha]-actinin/p27 , 2B p27 Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.
TS17: trunk paraxial mesenchyme Present Regionally restricted 2B [alpha]-actinin/p27 , 2B p27 Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm.

Specimen 2C p27 +/-: embryonic day 10.5; Cdkn1btm1Mlf/Cdkn1b+ (more )
Note: This specimen was coimmunostained with Myogenin (see Assay MGI:3611793).
Structure Level Pattern Image Note
TS17: trunk myotome Present 2C Myogenin/p27 +/-

Specimen 2C p27 -/-: embryonic day 10.5; Cdkn1btm1Mlf/Cdkn1btm1Mlf (more )
Note: This specimen was coimmunostained with Myogenin (see Assay MGI:3611793).
Structure Level Pattern Image Note
TS17: trunk myotome Absent 2C Myogenin/p27 -/-

Specimen 3 p27 upper: embryonic day 10.5 (more )
Note: This specimen was coimmunostained with Myogenin (see Assay MGI:3611793).
Structure Level Pattern Image Note
TS17: trunk myotome Present Regionally restricted 3_E10.5 p27 , 3_E10.5 Myogenin/p27 Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. Only a subset of cells that were immunopositive were also positive for the expression of Myogenin (see Assay MGI:3611793).
TS17: trunk paraxial mesenchyme Present Regionally restricted 3_E10.5 p27 , 3_E10.5 Myogenin/p27 Expression was detected in forelimb level myotomal cells but no other cells in the paraxial mesoderm. Only a subset of cells that were immunopositive were also positive for the expression of Myogenin (see Assay MGI:3611793).

Specimen 3 p27 lower: embryonic day 11.5 (more )
Note: This specimen was coimmunostained with Myogenin (see Assay MGI:3611793).
Structure Level Pattern Image Note
TS19: trunk myotome Absent 3_E11.5 p27 , 3_E11.5 Myogenin/p27
TS19: nervous system Strong 3_E11.5 p27 , 3_E11.5 Myogenin/p27
TS19: dorsal root ganglion Strong Regionally restricted 3_E11.5 p27 , 3_E11.5 Myogenin/p27 Expression was strong in dorsal root ganglia adjacent to myotome-derived muscle.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/23/2021
MGI 6.17
The Jackson Laboratory