Probe:	UI-M-CC0-ayf-c-01-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	This data is from Supplemental Table 5, Gene column, row: 250919 necdin. After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Present	Regionally restricted		Expression was detected in the inner neuroblastic layer in the central retina.

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted		Expression was detected in the inner neuroblastic layer and a subset of cells in the outer neuroblastic layer. The expression level was higher in the inner neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Present	Regionally restricted	Submitted Image - AF56e16.5 , S10_necdin E16	Expression was detected in the inner neuroblastic layer and was weak in a subset of cells in the outer neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present		S10_necdin E18 , Submitted Image - AF56e18.5	Expression was detected in the inner neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Weak		Submitted Image - AF56p1.5 , S10_necdin P0	Expression was detected in the inner neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Weak		S10_necdin P2 , Submitted Image - AF56p3.5	Expression was detected in the inner neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Weak		S10_necdin P4	Expression was detected in the inner neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Weak	Regionally restricted	S10_necdin P6 , Submitted Image - AF56p6.5	Expression was detected in amacrine cells.
TS28: retina ganglion cell layer	Weak		S10_necdin P6 , Submitted Image - AF56p6.5

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	S10_necdin P8 , Submitted Image - AF56p8.5	Expression was detected in amacrine cells and a small subset of horizontal cells.
TS28: retina ganglion cell layer	Present		S10_necdin P8 , Submitted Image - AF56p8.5

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	Submitted Image - AF56adult , S10_necdin Adult	Expression was detected in a subset of amacrine cells.
TS28: retina ganglion cell layer	Present	Regionally restricted	Submitted Image - AF56adult , S10_necdin Adult	Expression was detected in a subset of ganglion cells.

Structure	Level	Pattern	Image	Note
TS28: central nervous system	Present			Expression was widespread.
TS28: cerebellum	Absent
TS28: cerebral cortex	Weak	Regionally restricted		Expression was low outside of layer 6 of the cortex.

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present
TS24: peripheral nervous system	Present
TS24: cerebral cortex	Absent