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Gene Expression Data
Assay Details
Assay
Reference: J:55464 Rivolta MN, et al., GATA3 is downregulated during hair cell differentiation in the mouse cochlea. J Neurocytol. 1998 Sep;27(9):637-47
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3575418
Gene symbol: Gata3
Gene name: GATA binding protein 3
Antibody: GATA-3 antibody
Detection system: Secondary antibody coupled to rhodamine
Assay notes: The fixation was in methanol/acetone.
Results
Specimen 1B: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cochlear duct epithelium Present Uniform 1A , 1B This area shows labeling in the basal turn.

Specimen 1C: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cochlear duct epithelium Present Uniform 1A , 1C This area shows labeling in the apical turn.

Specimen 2B: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: organ of Corti Present Regionally restricted 2B This view is of the epithelium of the apical turn.

Specimen 2D: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: organ of Corti Weak Regionally restricted 2D This view is of the basal turn. Labeling is in the epithelium and labeling in the hair cells has decreased.

Specimen 2E: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: organ of Corti Weak Regionally restricted 2E This view is of the basal turn. Labeling is in the epithelium and labeling in the hair cells has decreased.

Specimen 3A: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: organ of Corti Present Regionally restricted 3A This view is or the basal turn. The outer hair cells show little label, but the inner hair cells show positive labeling. There is strong labeling in phalangeal cells, Deiter's cells, pillar cells and Hensen's cells.

Specimen 4B: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: organ of Corti Present Single cells 4B In the basal turn, labeling intensity has decreased in all cells. This includes supporting cells in the organ of Corti, from the base to the apex of the cochlea. In the apical turn, hair cells were not labeled, while Deiter's cells remained positive.

Specimen 5A: postnatal day 14 (more )
Structure Level Pattern Image Note
TS28: organ of Corti Absent 5A Expression is absent in the entire epithelium.

Specimen 7B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cochlear duct epithelium Present Single cells 7B , 7A The spiral ganglion cells are positively labeled. There is a decrease in the labeling of the hair cells in the basal turn that is described in the text.

Specimen 8B: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: utricle epithelium Absent 8A , 8B

Specimen Not shown: postnatal day 6 (more )
Structure Level Pattern Image Note
TS28: organ of Corti Weak Single cells Only scattered cells remain positive.

Specimen 6 E15: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: cochlea Present Regionally restricted 6 Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 100% expressed Gata3 at E15.5.

Specimen 6 E14: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: cochlea Present Regionally restricted 6 Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 100% expressed Gata3 at E14.5.

Specimen 6 E16: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: cochlea Present Regionally restricted 6 Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 80% expressed Gata3 at E16.5.

Specimen 6 E 18: embryonic day 18.5 (more )
Structure Level Pattern Image Note
TS26: cochlea Weak Regionally restricted 6 Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 30% expressed Gata3 at E18.5.

Specimen 6 P0: postnatal day 0 (more )
Structure Level Pattern Image Note
TS27: cochlea Weak Regionally restricted 6 Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 15% expressed Gata3 at P0.

Specimen 6 P14: postnatal day 14.5 (more )
Structure Level Pattern Image Note
TS28: cochlea Absent 6 Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 0% expressed Gata3 at P14.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/12/2021
MGI 6.17
The Jackson Laboratory