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Gene Expression Data
Assay Details
Assay
Reference: J:33829 Makino Y, et al., Development of Valpha4+ NK T cells in the early stages of embryogenesis. Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6516-20
Assay type: RT-PCR
MGI Accession ID: MGI:3052155
Gene symbol: Tcra-V14
Gene name: T cell receptor alpha, variable 14
Probe: VC2, VC6
Probe preparation: labelled with P32
Visualized with: Phosphorimaging
Assay notes: Nested primer sets were used for this analysis. The outer set was MGI:3052140. The RT-PCR products were run on a gel, transferred to a membrane, and hybridized with a gene specific probe (MGI:3052148).
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
NC Control
d9.5 yolk sac E10.0 (a) TS16: yolk sac Absent 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
d9.5 embryo E10.0 (a) TS16: embryo Present 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
e11.5 yolk sac E12.0 (a) TS20: yolk sac Present 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
e11.5 liver E12.0 (a) TS20: liver Present 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
d13.5 yolk sac E14.0 (a) TS22: yolk sac Present 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
d13.5 thymus E14.0 (a) TS22: thymus primordium Absent 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
d13.5 liver E14.0 (a) TS22: liver Present 0.1 µg; total RNA involves: 129S7/SvEvBrd * BALB/c Rag1tm1Mom/Rag1tm1MomNot Specified
adult thymus P adult TS28: thymus Present 0.1 µg; total RNA Not Specified Not Specified
Notes:
(a) Age normalized so that noon of plug day = E0.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory